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  • Open Access

Immunomodulatory potential of probiotics oraly delivered with β- LG to Balb/C mice

  • 1,
  • 1 and
  • 1
Clinical and Translational Allergy20155 (Suppl 3) :P121

https://doi.org/10.1186/2045-7022-5-S3-P121

  • Published:

Keywords

  • Flow Cytometry
  • Time Increase
  • Cell Subset
  • Flow Cytometry Analysis
  • Humoral Response
Two commercial probiotics preparations were analysed regarding the possibility to induce different T cells population. Determining CD4+, CD4+CD8+ and CD8+ expression (Tab.1) one was subjected to further experiments and delivered it together with milk allergen β-LG to Balb/C mice. 14, 21, 28 and 35d from the beginning of experiments specific serum IgG&IgA, sIgA and copro-IgA were checked. We found significant decrease of humoral response in group fed with β-LG with probiotic compared to group with β-LG only, the same time increase specific copro-IgA by 35 days of experiments was observed (Fig. 1). Flow cytometry analysis of T cells subsets show significant decreased in percentage of CD8+ population in head and neck lymph nodes (HNLN) and spleen (SPL) when probiotic was delivered. In Peyer's Patches elevated number of CD4+CD8+ and CD8+ T cell subsets was observed in the same group. During in vitro studies lymphocytes isolated from group β-LG+probiotic show significant increased CD8+, CD8+CD4+ compare to B-LG group. Results show that probiotics has potential to modulate immune answer to food allergens.

Table 1

  

DICOFLOR

SD

PROACTIVE

SD

SPL

CD8

9.96

0.766

9.2

1.3

 

CD4CD8

0.276

0.146

1.19

0.567

 

CD4

23.9

1.62

21.7

0.808

PP

CD8

2.81

1.41

1.02

0.639

 

CD4CD8

0.26

0.149

0.609

0.914

 

CD4

12.2

4.7

3.19

0.75

HNLN

CD8

17.6

0.952

18.1

2.12

 

CD4CD8

1.26

0.534

0.323

0.203

 

CD4

47.5

3.01

43.4

2.83

MLN

CD8

13.2

1.59

14.8

1.53

 

CD4CD8

1.1

1.14

0.842

1.04

 

CD4

45.4

14.6

52.3

4.08

SERUM

CD8

11.7

1.01

9.18

0.479

 

CD4CD8

0.281

0.463

0.0554

0.0213

 

CD4

35.6

3.42

29

2.87

Figure 1
Figure 1

Specific antibodies titer

Declarations

Acknowledgements

The project was funded by the National Science Centre, decision DEC-2011/01/B/NZ9/02727.

Authors’ Affiliations

(1)
Department of Food Immunology and Microbiology, Division of Food Science, Institute of Animal Reproduction and Food Research of PAS, Olsztyn, Poland

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