Volume 5 Supplement 3

Abstracts from the Food Allergy and Anaphylaxis Meeting 2014

Open Access

A peanut quality control material to improve allergen analysis – How difficult can it be?

  • Michael Walker1,
  • Gill Holcombe1,
  • Deborah House1,
  • Joanna Topping1 and
  • Clare Mills2
Clinical and Translational Allergy20155(Suppl 3):P116

https://doi.org/10.1186/2045-7022-5-S3-P116

Published: 30 March 2015

Flawed allergen analysis hinders detection and measurement of allergens, which is important for allergen risk management in food labelling, in-factory cleaning and threshold levels. ELISA, DNA and MS can be used to detect food allergens but there are quantification problems with all three. ELISA is currently the most commonly applied technique however, although it can detect the presence of most protein allergens in foods, it can struggle with recovery and accurate quantification. A well characterised quality control material, ideally a certified reference material, has been called for by many to improve consistency in analytical results for allergens. However there are practical difficulties with production which are often overlooked, including ensuring sufficient homogeneity and long term stability, assessing the incurred quantity, and maintaining a relationship with the concentrations that affect allergy sufferers. The authors present a successful study which is a first step towards addressing the above problems. A prototype quality control set, (a blank material and a QC material with peanut protein added at 10 mg kg-1 level) has been prepared based on a EuroPrevall study matrix used to assess clinical thresholds[1]. The homogeneity and stability of the material have been evaluated and assessed as satisfactory and the material is now available to the wider analytical community. This is valuable progress in addressing the lack of allergen related incurred reference materials to improve global allergen protein measurement.

Authors’ Affiliations

(1)
LGC
(2)
Manchester University

References

  1. Cochrane X, et al: Development of a standardized low-dose double-blind placebo-controlled challenge vehicle for the EuroPrevall project. Allergy. 2012, 67: 107-113. 10.1111/j.1398-9995.2011.02715.x.View ArticlePubMedGoogle Scholar

Copyright

© Walker et al; licensee BioMed Central Ltd. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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