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Effects of atopy and rhinitis on exhaled nitric oxide values - a systematic review
Clinical and Translational Allergyvolume 1, Article number: 8 (2011)
Atopy and rhinitis are among the factors affecting exhaled nitric oxide (FeNO) values and may contribute to difficulties in the clinical interpretation of FeNO measurements. However, data assessing their effects on FeNO values had never been summarized. This review aims to evaluate the effect of atopy and rhinitis in FeNO values in otherwise healthy individuals.
A systematic review was performed in Pubmed, Scopus and ISI Web of Knowledge. A two-step selection process was completed, and from 2357 references 19 were included. The inclusion criteria were: participants without known diseases other than rhinitis; atopy assessement by SPT or Specific IgE; and FeNO measurements according to ATS/ERS recommendations.
The 8 articles measuring FeNO in children showed higher values in both allergic rhinitis and atopic children when compared with healthy children. The 11 articles performed in adults observed higher FeNO in AR patients comparatively with either healthy or atopic individuals. However, adult healthy and atopic individuals had similar FeNO values.
FeNO values are higher in individuals with rhinitis and/or atopy without other health problems. These effects are small, seem to be independent and should be further studied using multivariate models. The effect of atopy was observed only in children. The combined effect of atopy and rhinitis produced higher FeNO values in adults. These results support that both atopy and rhinitis should be considered when interpreting or when defining FeNO reference values.
Exhaled nitric oxide (FeNO) is associated with asthma , and with airway eosinophilia . Recently, it was suggested that FeNO production in the airways is under the influence of Th2 cytokines, IL-4 and IL-13, responsible for induction of Inducible Nitric Oxide Synthase (iNOS) expression in the airway epithelium, and thus for the increase of FeNO in inflammatory disorders .
Many individual factors influence FeNO values. In fact, the considerable inter-subject variability hampers the clinical interpretation of FeNO measurements [4, 5]. Two important FeNO modifiers are atopy and rhinitis . Some studies reported higher FeNO values in patients with atopy or allergic rhinitis alone [7–9], while others observed a relationship between elevated FeNO values and the exacerbation of rhinitis and the number of positive wheals on skin prick tests (SPT) [10, 11]. However, results are controversial, as there are studies that did not observe significant differences of FeNO values in atopy or rhinitis [12–14].
The aim of this systematic review was to evaluate the effect of atopy and rhinitis in FeNO values in otherwise healthy individuals.
This systematic review follows the PRISMA recommendations . Three databases were searched: Pubmed, Scopus and ISI Web of Knowledge. The limits used were a) English language, b) publication date from 1990 to October 2009, and c) studies with original data. The main search terms were "FeNO", "Rhinitis, "Atopy" and equivalent expressions.
The inclusion criteria were: 1) atopy assessed by Skin Prick Tests (SPT) or specific IgE for common aeroallergens; 2) online FeNO measurements, according to the American Thoracic Society/European Respiratory Society (ATS/ERS) recommendations ; 3) study participants of at least two of the following groups: healthy, atopic, with allergic rhinitis or non allergic rhinitis. Exclusion criteria were 1) in vitro or animal experiments or 2) participants with known diseases other than rhinitis, e.g. asthma alone. A study group was defined as atopic in the presence of allergic sensitization without a medical diagnosis of asthma and rhinitis. Study selection had two phases (Figure 1). In Phase 1, two reviewers screened the titles and abstracts independently. If one of the reviewers included the abstract, it was allowed into the Phase 2. In Phase 2, manuscripts were analyzed independently and disagreements were discussed between reviewers
Data was extracted using an electronic form developed by the authors. Information on characteristics of the included studies and their samples was summarized in tables 1 and 2 and Additional File 1 (tables S1-S2). Six studies did not present FeNO values. Authors were contacted for the FeNO data, but no answer was obtained. Four of these studies had FeNO values comparisons and were included, and the remaining 2 were excluded.
Two groups of studies were defined, based on the participants' age - less than 18 years old and adults. In each group, the studies were divided according to the summary measure used for reporting FeNO values. Four groups of FeNO values were defined: Allergic Rhinitis (AR), Non-Allergic Rhinitis (NAR), Healthy and Atopic. Values were compared in sets of 2, with a total of 6 comparisons. A ratio of FeNO values in each comparison is presented. One study provided results during and off pollen season . Both results were included, in separate conditions. When data allowed it, meta-analysis were done using Review Manager 5 (Cochrane Collaboration)  using a random effects model, due to differences on studies methods. Results showed high heterogeneity (I2) and were not included for analysis (Additional File 2, figures S1-S3).
Articles search retrieved a total of 2357 references after duplicates' removal. In the first selection phase 2239 articles were excluded, mainly studies with patients with known conditions other than rhinitis (Figure 1). In the second selection phase, all full-text articles were retrieved and analyzed. Nineteen studies were included in the systematic review (Figure 1), in eight the participants were children and in 11 were adults. Most were cross sectional studies (n = 16), and 3 were prospective. Seven did not report the study setting, 8 recruited the subjects from outpatient clinics, 2 from schools, one from general population and another one from mills (Table 1). Sample sizes ranged from 20 to 276 participants (Table 1). Three studies included smokers and 9 included only non-smokers individuals; the remaining 7 studies did not report smoking status (Additional File 1, Table S1); one of the studies with children  excluded those that were exposed to tobacco smoke in the family.
Most studies used SPT to identify atopic patients (Table 1). Rhinitis was defined based on symptoms (n = 9) or clinical assessment (n = 5) (Table 1). The definition of healthy individuals was not reported in three studies, and all the others excluded atopic individuals (Additional File 1, Table S2).
Comparisons between defined groups were reported in all studies, but in 4 these comparisons did not present numerical values (Table 2). Seven articles compared Atopic and Healthy individuals. The 3 studies performed in adults showed no significant differences in FeNO values, while in children, the studies showed higher FeNO values in the atopic groups. Sixteen articles compared FeNO values between Allergic Rhinitis and Healthy individuals in a total of 17 comparisons; 12 showed significant higher values of FeNO in the AR patients. Two studies compared Atopic and Non-Allergic Rhinitis individuals: one performed in children observed a non-significant higher in FeNO values in atopic individuals, while another, with an adult population, observed lower values. When comparing Allergic Rhinitis and Atopic individuals all the 4 studies (2 in children) showed higher FENO values in allergic rhinitis; in 2 of them the values were significantly higher and in the other the statistical significance was not reported. All articles comparing Allergic Rhinitis and Non-Allergic Rhinitis subjects (n = 3) showed higher FeNO values in allergic rhinitis patients. From the three articles comparing FeNO values between Non-Allergic Rhinitis individuals and Healthy ones, 2 had no significant differences, and one showed higher values in the NAR group (Figure 2).
The collected data was insufficient for quantitative synthesis. For each comparison, 2 to 3 studies could be used, and 2 out of the 3 meta-analysis had high heterogeneity (I2 = 100%) (Additional File 2, Figures S1-S3).
This is the first systematic review that summarizes the influence of Allergic Rhinitis and Atopy on FeNO values. FeNO was higher in children with atopy and in children with allergic rhinitis, when compared with children without rhinitis, atopic or with NAR. In adults, a similar increase was observed with AR, but FeNO values were similar between atopic and healthy individuals. These results are in agreement with previous non-systematic reviews on AR effect on nasal and/or exhaled nitric oxide values [5, 18]. Previous results also support the consistent effect of atopy on FeNO values that we observed in healthy children; however they are not in agreement with the absence of effect of atopy in FeNO levels that we found in adults [5, 19]. Similar values were also observed between atopic individuals without rhinitis and patients with NAR and with NAR and healthy children. However, only 3 studies with patients with NAR were available.
This systematic review supports the argument that atopy and rhinitis should be considered for the clinical interpretation of FeNO measurements. In fact, adults with allergic rhinitis and children with atopy (those presenting higher FeNO levels) may have FeNO measurements out of the 'normal' range previously proposed that do not take in account both atopy and rhinitis. To allow a visual representation of comparisons between groups we present the illustration in Figure 2 [20, 21].
Our study has some limitations. The interpretation of quantitative synthesis of data was not possible. FeNO values were presented in different ways (e.g. different summary and dispersion measures) (Table 2) so only 2 to 3 articles could be used for each comparison. Moreover, a high heterogeneity was observed (Additional File 2, Figure S1-S3). Despite our best efforts, we had no access to the values of FeNO in four studies. These articles were included on the review, allowing a broad qualitative assessment of published studies but could not be used in the meta-analysis.
The methods of the included studies were highly variable. There were variations in the definitions of atopy, rhinitis and healthy individuals. The definition of healthy individuals was particularly variable, often incomplete and sometimes missing. Also, various settings, eligibility criteria and sample sizes were used. In some of the studies with children [8, 12, 21–23] the age range was too broad (> 5 years). FeNO in children is strongly associated with age and body size . The inclusion criteria regarding smoking status were different and many did not report the smoking status (Additional File 1, Table S1). As smoking [9, 24–26], and passive smoking , can interfere with FeNO values, not accounting for the smoking status may be one of the reasons for the differences observed between the studies. Moreover, most studies presented only univariate analysis, with few multivariate models reported. Multivariate models could consider additional personal characteristics (e.g. height, age or gender).
Future studies should report adequate summary and dispersion measures, allowing for further quantitative synthesis. Samples sizes and subjects characteristics should be accurately described. Moreover, the factors that are known to modify FeNO should be taken into account. It is also important to follow current recommendations on FENO measurement .
In conclusion, FeNO values are higher in individuals with rhinitis and/or atopy without other diseases. These effects are small, seem to be independent and should be further studied using multivariate models. The causes for the effects of atopy and rhinitis on FENO values also need further study. The effect of atopy was observed only in children. The combined effect of atopy and rhinitis produced higher FeNO values in adults. These results support that atopy and rhinitis should be considered when interpreting or when defining FeNO reference values.
American Thoracic Society/European Respiratory Society
inducible Nitric Oxide Synthase
Skin Prick Tests.
Alving K, Weitzberg E, Lundberg JM: Increased amount of nitric oxide in exhaled air of asthmatics. Eur Respir J. 1993, 6: 1368-1370.
Payne DN, Adcock IM, Wilson NM, Oates T, Scallan M, Bush A: Relationship between exhaled nitric oxide and mucosal eosinophilic inflammation in children with difficult asthma, after treatment with oral prednisolone. Am J Respir Crit Care Med. 2001, 164: 1376-1381.
Alving K, Malinovschi A: Basic aspects of exhaled nitric oxide. Exhaled Biomarkers. 2010, European Respiratory Society Journals Ltd, 49: 1-31.
Jiang J, Malavia N, Suresh V, George SC: Nitric oxide gas phase release in human small airway epithelial cells. Respir Res. 2009, 10: 3-10.1186/1465-9921-10-3.
Abba A: Exhaled nitric oxide in diagnosis and management of respiratory diseases. Annals of Thoracic Medicine. 2009, 4: 173-181. 10.4103/1817-1737.56009.
ATS/ERS statement: raised volume forced expirations in infants: guidelines for current practice. Am J Respir Crit Care Med. 2005, 172: 1463-1471. 10.1164/rccm.200408-1141ST.
Scott M, Raza A, Karmaus W, Mitchell F, Grundy J, Kurukulaaratchy RJ, Arshad SH, Roberts G: Influence of atopy and asthma on exhaled nitric oxide in an unselected birth cohort study. Thorax. 2010, 65: 258-262. 10.1136/thx.2009.125443.
Hervas D, Milan JM, Garde J: Differences in exhaled nitric oxide in atopic children. Allergol Immunopathol (Madr). 2008, 36: 331-335. 10.1016/S0301-0546(08)75865-8.
Travers J, Marsh S, Aldington S, Williams M, Shirtcliffe P, Pritchard A, Weatherall M, Beasley R: Reference ranges for exhaled nitric oxide derived from a random community survey of adults. Am J Respir Crit Care Med. 2007, 176: 238-242. 10.1164/rccm.200609-1346OC.
Franklin PJ, Taplin R, Stick SM: A community study of exhaled nitric oxide in healthy children. American Journal of Respiratory and Critical Care Medicine. 1999, 159: 69-73.
Cardinale F, de Benedictis FM, Muggeo V, Giordano P, Loffredo MS, Iacoviello G, Armenio L: Exhaled nitric oxide, total serum IgE and allergic sensitization in childhood asthma and allergic rhinitis. Pediatr Allergy Immunol. 2005, 16: 236-242. 10.1111/j.1399-3038.2005.00265.x.
Cibella F, Cuttitta G, La Grutta S, Passalacqua G, Viegi G: Factors that influence exhaled nitric oxide in Italian schoolchildren. Annals of Allergy, Asthma and Immunology. 2008, 101: 407-412. 10.1016/S1081-1206(10)60318-3.
Profita M, La Grutta S, Carpagnano E, Riccobono L, Di Giorgi R, Bonanno A, Pace E, Bonsignore G, Bousquet J, Vignola AM, Gjomarkaj M: Noninvasive methods for the detection of upper and lower airway inflammation in atopic children. J Allergy Clin Immunol. 2006, 118: 1068-1074. 10.1016/j.jaci.2006.07.028.
Berlyne GS, Parameswaran K, Kamada D, Efthimiadis A, Hargreave FE: A comparison of exhaled nitric oxide and induced sputum as markers of airway inflammation. J Allergy Clin Immunol. 2000, 106: 638-644. 10.1067/mai.2000.109622.
Liberati A, Altman DG, Tetzlaff J, Mulrow C, Gøtzsche PC, Ioannidis JPA, Clarke M, Devereaux PJ, Kleijnen J, Moher D: The PRISMA statement for reporting systematic reviews and meta-analyses of studies that evaluate health care interventions: Explanation and elaboration. Italian Journal of Public Health. 2009, 6: 354-391.
Prieto L, Uixera S, Gutiérrez V, Bruno L: Modifications of airway responsiveness to adenosine 5'-monophosphate and exhaled nitric oxide concentrations after the pollen season in subjects with pollen-induced rhinitis. Chest. 2002, 122: 940-947. 10.1378/chest.122.3.940.
Review Manager (RevMan) [Computer Program]. Book Review Manager (RevMan) [Computer Program]. 2008, City: The Nordic Cochrane Centre, The Cochrane Collaboration, 5.0
Maniscalco M, Sofia M, Pelaia G: Nitric oxide in upper airways inflammatory diseases. Inflamm Res. 2007, 56: 58-69. 10.1007/s00011-006-6111-1.
Payne DN: Nitric oxide in allergic airway inflammation. Curr Opin Allergy Clin Immunol. 2003, 3: 133-137. 10.1097/00130832-200304000-00007.
Olin AC, Bake B, Toren K: Fraction of exhaled nitric oxide at 50 mL/s: reference values for adult lifelong never-smokers. Chest. 2007, 131: 1852-1856. 10.1378/chest.06-2928.
Malmberg LP, Petays T, Haahtela T, Laatikainen T, Jousilahti P, Vartiainen E, Makela MJ: Exhaled nitric oxide in healthy nonatopic school-age children: determinants and height-adjusted reference values. Pediatr Pulmonol. 2006, 41: 635-642. 10.1002/ppul.20417.
Hung CH, Hua YM, Hsu WT, Lai YS, Yang KD, Jong YJ, Chu YT: Montelukast decreased exhaled nitric oxide in children with perennial allergic rhinitis. Pediatr Int. 2007, 49: 322-327. 10.1111/j.1442-200X.2007.02375.x.
Marcucci F, Passalacqua G, Canonica GW, Frati F, Salvatori S, Di cara G, Petrini I, Bernini M, Novembre E, Bernardini R, Incorvaia C, Sensi LG: Lower airway inflammation before and after house dust mite nasal challenge: an age and allergen exposure-related phenomenon. Respir Med. 2007, 101: 1600-1608. 10.1016/j.rmed.2006.06.006.
Malinovschi A, Janson C, Holmkvist T, Norback D, Merilainen P, Hogman M: IgE sensitisation in relation to flow-independent nitric oxide exchange parameters. Respir Res. 2006, 7: 92-10.1186/1465-9921-7-92.
Yates DH: Role of exhaled nitric oxide in asthma. Immunol Cell Biol. 2001, 79: 178-190. 10.1046/j.1440-1711.2001.00990.x.
Sundy JS, Hauswirth DW, Mervin-Blake S, Fernandez CA, Patch KB, Alexander KM, Allgood S, McNair PD, Levesque MC: Smoking is associated with an age-related decline in exhaled nitric oxide. European Respiratory Journal. 2007, 30: 1074-1081. 10.1183/09031936.00087807.
Laoudi Y, Nikasinovic L, Sahraoui F, Grimfeld A, Momas I, Just J: Passive smoking is a major determinant of exhaled nitric oxide levels in allergic asthmatic children. Allergy. 2010, 65: 491-497. 10.1111/j.1398-9995.2009.02190.x.
Saito J, Inoue K, Sugawara A, Yoshikawa M, Watanabe K, Ishida T, Ohtsuka Y, Munakata M: Exhaled nitric oxide as a marker of airway inflammation for an epidemiologic study in schoolchildren. J Allergy Clin Immunol. 2004, 114: 512-516. 10.1016/j.jaci.2004.05.033.
Prieto L, Gutiérrez V, Uixera S: Exhaled nitric oxide and bronchial responsiveness to adenosine 5'-monophosphate in subjects with allergic rhinitis. Chest. 2002, 121: 1853-1859. 10.1378/chest.121.6.1853.
Rolla G, Guida G, Heffler E, Badiu I, Bommarito L, De Stefani A, Usai A, Cosseddu D, Nebiolo F, Bucca C: Diagnostic classification of persistent rhinitis and its relationship to exhaled nitric oxide and asthma: a clinical study of a consecutive series of patients. Chest. 2007, 131: 1345-1352. 10.1378/chest.06-2618.
Rouhos A, Kainu A, Karjalainen J, Lindqvist A, Piirila P, Sarna S, Haahtela T, Sovijarvi ARA: Atopic sensitization to common allergens without symptoms or signs of airway disorders does not increase exhaled nitric oxide. Clinical Respiratory Journal. 2008, 2: 141-148. 10.1111/j.1752-699X.2007.00045.x.
Kostikas K, Papaioannou AI, Tanou K, Koutsokera A, Papala M, Gourgoulianis KI: Portable exhaled nitric oxide as a screening tool for asthma in young adults during pollen season. Chest. 2008, 133: 906-913. 10.1378/chest.07-1561.
Tanou K, Koutsokera A, Kiropoulos TS, Maniati M, Papaioannou AI, Georga K, Zarogiannis S, Gourgoulianis KI, Kostikas K: Inflammatory and oxidative stress biomarkers in allergic rhinitis: the effect of smoking. Clin Exp Allergy. 2009, 39: 345-353. 10.1111/j.1365-2222.2008.03149.x.
Gratziou C, Rovina N, Makris M, Simoes DCM, Papapetropoulos A, Roussos C: Breath markes of oxidative stress and airway inflammation in seasonal allergic rhinitis. International Journal of Immunopathology and Pharmacology. 2008, 21: 949-957.
Olin AC, Alving K, Torén K: Exhaled nitric oxide: Relation to sensitization and respiratory symptoms. Clinical and Experimental Allergy. 2004, 34: 221-226. 10.1111/j.1365-2222.2004.01888.x.
Aronsson D, Tufvesson E, Bjermer L: Allergic rhinitis with or without concomitant asthma: difference in perception of dyspnoea and levels of fractional exhaled nitric oxide. Clin Exp Allergy. 2005, 35: 1457-1461. 10.1111/j.1365-2222.2005.02363.x.
Mansson A, Bachar O, Adner M, Cardell LO: Nasal CpG oligodeoxynucleotide administration induces a local inflammatory response in nonallergic individuals. Allergy. 2009, 64: 1292-1300. 10.1111/j.1398-9995.2009.02012.x.
We thank Alexandra Caeiro, Ana Coelho, Ana Teresa Abreu, Bruna Guedes, Gisela Vasconcelos, Inês Pita, João Alberto Fonseca, Maria Gomez, Marta Oliveira, Vítor Abreu for participating in a previous work on the same topic done as an assignment for Introdução à Medicina course at Faculdade de Medicina da Universidade do Porto in 2008/2009 and Altamiro Costa Pereira and Luís Nogueira-Silva for their input during that work.
DL and AMP declare no competing interests. JAF and TJ received an unrestricted grant from Aerocrine for the development of a FeNO interpretation aid tool (http://feno.med.up.pt).
DL, TJ, JAF contributed to the design of the study, acquisition, interpretation and analysis of data and in the manuscript draft and review. AMP participated in the acquisition and interpretation of the data and in the manuscript draft and review. All authors read and approved the final manuscript.