Fig. 5

IgE binding activity of N-terminal epitope of Der p 24 and main linear IgE epitopes of Der p 1 or Der p 2. a Indirect IgE-ELISA with serum samples from HDM-sensitized patients (N = 30) and non-allergic controls (N = 2). b IgE-dot blot analysis with sera from HDM allergic patients (N = 8, P1–P8) and non-allergic individuals (N = 2, N1–N2). c Indirect IgE-ELISA for detecting specific IgE against immunodominant IgE epitopes of Der p 24 with individual serum samples from HDM allergic patients with symptom manifestations of only asthma, rhinitis, urticaria, or dermatitis (N = 10 per manifestation group). Mean values are shown with standard errors and p values < 0.05 were considered significant