Fig. 3

Identification of N-terminal 32 residues of Der p 24 is an immunodominant IgE epitope. a Synthesized overlapping polypeptides designed according to the N-terminal 32 residue sequences and conjugated with BSA protein for comparative analysis of IgE binding reactivity. b IgE-ELISAs of overlapping polypeptides with individual sera from 15 HDM-allergic patients. P values < 0.05 were considered significant. c IgE-dot blot of overlapping polypeptides (2 μg each) incubated with sera from 5 HDM-allergic patients (P1–P5; subset from experiment in B with positive reactions) and pooled sera from 3 non-allergic controls (N). d IgE-western blot of BSA-Der p 24(1–32aa) polypeptide conjugate with serum samples from 8 HDM-sensitized patients (P1–P8) and 3 non-allergic individuals (N1–N3). e Homology-based three-dimensional model of the structure of Der p 24, which included two alpha helices and two random coils. The N-terminal-32 residue region is labelled red