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Table 1 HPLC analytical parameters for MT

From: Interchangeability and comparative effectiveness between generic and brand montelukast immediate release tablets after a single oral administration in healthy volunteers

Mobile phase Buffer solution: dissolve 1.36 g potassium dihydrogen phosphate in 1 l and adjust PH to 4.0 with 20 % v/v phosphoric acid
Mix 1400 mL acetonitrile with 600 mL of buffer solution and filter through 0.45 µm membrane filter
Diluent Mobile phase
HPLC Waters system
Column Inertsil ODS-2 (25 cm × 4.6 mm) 5 μm
Detector 355 nm
Flow rate 1.5 mL/min
Injection volume 50 µL
Column oven temperature 40 °C
Sample cooler temperature NA
Standard solution preparation Standard solution contains: 0.02 mg/mL of montelukast
Sample solution preparation Standard solution contains: 0.02 mg/mL of montelukast
Run time 12 min
Filter Nylon filter is suitable after discarding the first 3 mL
PTFE filter is suitable after discarding the first 3 mL