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The use of recombinant fish parvalbumin Gad c 1 in the characterisation of fish allergic patients


IgE mediated allergy to fish is a cause of severe anaphylatic reactions. Parvalbumins are considered major fish allergens and also responsible for cross-reactivity.


Evaluation of tolerance acquisition using the recombinant fish parvalbumin (Gad c 1) in a group of fish allergic patients (pts).

Material and methods

We selected 55 pts (34M, 21F; average age:7.4 years). All had fish allergy, characterized by positive clinical history, skin-prick tests and serum specific IgE to several fish and Gad c 1 (UniCap®, Thermo-Fisher). Oral food challenge was performed to evaluate fish tolerance acquisition. Statistical analysis was performed using GraphPad Prisma version 5.0 (GraphPad Software Inc, SD). Two groups were compared using a paired t test Wilcoxon. P values<0.05 were considered significant.


The 55 fish allergic pts had clinical symptoms for more than one fish species: 43 (78%) pts had skin manifestations, 12 (22%) anaphylaxis, 8 (15%) respiratory and 9 (16%) gastrointestinal symptoms. Pts who suffered anaphylaxis had lower Gad c 1 average values (31 KU/L) than the others (76.7 KU/L); p=0.15. 38/55 (69%) pts were intolerant and 17/55 (31%) pts tolerated at least one fish species. Gad c 1 average values were significantly higher (21.6 KU/L) in the intolerant group than in the partially tolerant group (2.7 KU/L); p=0.009. 24/38 pts were followed for at least one year, on average 3.7 years. 14/24 pts developed fish tolerance to at least one fish species-Group I (10M, 4F; average age 7 years) and 10/24 were intolerant-Group II (6M, 4F; average age 7 years). Group I showed lower average values (4.6 KU/L) of Gad c 1 than the Group II (25.1 KU/L); p=0.0001.


Lower Gad c 1 values seem to be an important predictive parameter in fish tolerance acquisition but not in predicting clinical symptoms’ severity and might be considered a useful tool in the follow-up of fish allergic patients.

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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

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Cabral Duarte, F., Costa, A.C., Pereira Barbosa, M.A. et al. The use of recombinant fish parvalbumin Gad c 1 in the characterisation of fish allergic patients. Clin Transl Allergy 5 (Suppl 3), P22 (2015).

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