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Maillard-type neoallergens present in processed soy extract may cause an allergic reaction in soy allergic patients


Soybean is an important food allergen and it is often incorporated in complex formulated foods. Thermal processing induces conformational changes of food allergens and accelerates its interaction with carbohydrates. This reaction is called Maillard reaction (MR) and occurs always during food processing. It has been shown to influence allergenic potential of several proteins.


The aim of the study was to establish the effect of processing conditions on allergenic potential of soy protein extract containing the major soy allergens Gly m 5 and 6.

Material and methods

Clinically characterized soy allergic patients (n=15) with specific IgE against Gly m 5 and 6 were selected for the study. Here we show the complete data obtained for seven patients. Five chemically characterized soy protein extracts (SPE) were tested: raw SPE, SPE heated (121oC) for 10 or 30 minutes with glucose and SPE heated (121oC) for 10 or 30 minutes without sugar as a control. The allergenic potential of tested extract was examined using the basophil activation test (BAT) and inhibition ELISA. Maillard reaction products (MRP) were identified by western blot.


Patient-dependent differences in the levels of activated basophils were seen in BAT test. When compared to raw soy an increase (from 2 to 5 times; 2 patients) or decrease (from 2 to 36 times; 3 patients) of allergenicity for all processed extracts was seen. For 2 patients miscellaneous changes for different processing conditions were observed. For 70% of the patients SPE heated for 30 minutes with glucose stimulated basophils stronger than control SPE. The neo-allergens formed during heating of SPE with glucose were characterized on blots as high-molecular weight agglomerates of proteins and sugars, which contain advanced MRP. Their inhibitory ability for specific IgE binding was established in ELISA.


Maillard-type neoallergens formed during soy processing can trigger stronger basophil stimulation than raw soy proteins in soy allergic patients. The use of only raw soy extracts in diagnostic tests may provide false negative results.


Supported by a Marie Curie Fellowship EU-FP7-PEOPLE-2011-IEF (302295).

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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

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Teodorowicz, M., Jansen, A.P.H., Roovers, M.H.W.M. et al. Maillard-type neoallergens present in processed soy extract may cause an allergic reaction in soy allergic patients. Clin Transl Allergy 5 (Suppl 3), P21 (2015).

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