- Poster presentation
- Open Access
Immunomodulatory effects of ovalbumin hydrolysates in a mouse model of food allergy
Clinical and Translational Allergy volume 5, Article number: P118 (2015)
Peptides released from enzymatic hydrolysis of certain proteins could exert immumodulatory effects on disorders such as food allergy. Peptide-based vaccines, corresponding to T cell epitopes of particular allergens, may retain immunogenicity, while being of insufficient length to cross-link allergen specific IgE on the surface of effector cells to elicit an allergic response. Ovalbumin (OVA) hydrolysates are a rich source of biologically bioactive peptides, some of which may present stimulatory activities on immune functions. On the other hand, cell cultures derived from mouse models of food allergy have proved very helpful in the evaluation of the allergenic and immunomodulatory abilities of hydrolysates and pure peptides. The aim of this study was to evaluate OVA hydrolysates as allergenic immune modulators by using splenocyte cultures of OVA-sensitized mice. The final goal is the development of new forms of immunotherapy, safer and more effective than those using whole allergens.
OVA was hydrolyzed with commercial enzymes (pepsin, Alcalase and Neutrase) and their products size-fractionated (3 kDa and 10 kDa). The hydrolysates and their fractions were characterized by SDS-PAGE, RP-HPLC and HPLC-MS/MS, and their IgE-binding properties were evaluated by inhibition ELISA using sera of egg-allergic patients. Balb/c mice were sensitized to OVA by the oral administration of the protein with cholera toxin. At the end point, mice splenocytes were isolated and cultured in presence of the hydrolysates and their fractions alone, or together with the sensitizing protein. The immunomodulatory effect of the hydrolysates was based on the cytokine profile, as determined by ELISA.
In splenocyte cultures incubated in presence of the hydrolysates and their fractions, a marker down-regulation of Th2-biased cytokines (IL-4, IL-5 and IL-13) was observed. When samples were stimulated with OVA, the 10 kDa-fractions showed a more pronounced reduction of Th2 cytokines, as compared with the parent hydrolysates. The levels of Th1-biased cytokines (IFN-γ and TNF-α) increased in the presence of the hydrolysates with Alcalase and Neutrase. The hydrolysates produced with Neutrase significantly increased the levels of regulatory IL-10, even in the presence of the allergen.
Hydrolysates of OVA with Neutrase exhibited an effective immunomodulatory effect in the cytokine profile of OVA-sensitized mice splenocytes. This might represent a novel immunotherapeutical approach in the treatment of egg allergy.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.
The Creative Commons Public Domain Dedication waiver (https://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
About this article
Cite this article
Lozano-Ojalvo, D., López-Expósito, I., Molina, E. et al. Immunomodulatory effects of ovalbumin hydrolysates in a mouse model of food allergy. Clin Transl Allergy 5 (Suppl 3), P118 (2015). https://doi.org/10.1186/2045-7022-5-S3-P118
- Food Allergy
- Cholera Toxin
- Immunomodulatory Effect
- Cytokine Profile