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  • Oral presentation
  • Open Access

The roles of basophils, TSLP and IL-33 in food allergy following epicutaneous sensitisation

  • 1,
  • 1,
  • 1 and
  • 1
Clinical and Translational Allergy20155 (Suppl 3) :O17

https://doi.org/10.1186/2045-7022-5-S3-O17

  • Published:

Keywords

  • Food Allergy
  • Oral Challenge
  • Food Antigen
  • Foxp3 mRNA
  • Thymic Stromal Lymphopoietin

Cutaneous sensitization with a food antigen before its consumption elicits the development of food allergy. Here we report the site and stage dependent roles of basophils and pro-allergic cytokines, thymic stromal lymphopoietin (TSLP) and IL-33, in a mouse model of food allergy initially sensitized cutaneously with the food antigen. Mice are epicutaneously sensitized with the food antigen ovalbumin (OVA) followed by oral challenge with OVA. Epicutaneously-sensitized mice produce OVA-specific IgE and develop IgE-dependent anaphylaxis after oral challenge. If OVA is given orally before epicutaneous administration, development of food allergy is prevented with Foxp3 mRNA up-regulation. Thus, our mouse model clearly reflects the “dual-allergen-exposure hypothesis” in which allergic sensitization results from cutaneous food antigen exposure before its consumption. When allergy is induced by epicutaneous sensitization then oral challenge, basophil-depleted or TSLP-receptor-deficient mice do not produce OVA-specific IgE and are protected from oral challenge-induced anaphylaxis. IL-33-deficient mice produce normal levels of OVA-specific IgE, however, IL-33-deficient mice and mice treated with recombinant soluble IL-33 receptor are protected from anaphylaxis. Thus, basophils and TSLP have pivotal roles in Th2 development in the skin during the sensitization phase of food allergy. In contrast, while IL-33 is dispensable for promoting cutaneous antigen sensitization, the cytokine is essential for inducing IgE-dependent anaphylaxis in the gut.

Authors’ Affiliations

(1)
Hyogo College of Medicine, Nishinomiya, Japan

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