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High-dose intravenous immunoglobulin therapy for eosinophilic granulomatosis with polyangiitis
Clinical and Translational Allergy volume 4, Article number: 38 (2014)
Regulatory T (Treg) cells are implicated in the development and progression of eosinophilic granulomatosis with polyangiitis (EGPA). We previously showed beneficial effects of intravenous immunoglobulin (IVIG) therapy combined with corticosteroid and immunosuppressant treatment on clinical symptoms, including mononeuritis multiplex and cardiac dysfunction, and Treg cell frequency, during EGPA. Whether the timing of administration (during initial treatment or at relapse after remission) or previous treatment affects the clinical and immunologic efficacy of IVIG is unknown. We evaluated whether the frequency of Treg cells varied depending on when IVIG was provided relative to the start of conventional therapy for EGPA.
The patient population for this retrospective analysis comprised 17 patients with severe mononeuritis multiplex or heart failure whose EGPA did not respond to corticosteroids combined with immunosuppressant therapy. Ten patients first received IVIG during initial treatment, whereas the remaining 7 patients first received IVIG on relapse after remission. We measured the percentage of Treg cells, defined as FOXP3+CD4+ T cells, present before the first round of IVIG and at 1 month after the last IVIG treatment.
FOXP3+CD4+ T cells were increased in patients who required only a single course of IVIG to achieve remission compared with those who needed two or more courses. The dosage of prednisolone at initial IVIG was inversely correlated with the ratio of the number of FOXP3+CD4+ T cells before IVIG and that at 1 month thereafter.
Patients with severe EGPA who receive IVIG after nonresponse to high-dose prednisolone during initial treatment may need multiple courses of IVIG to achieve remission. An increase in the frequency of Treg cells after IVIG may predict the need for additional IVIG in EGPA.
Eosinophilic granulomatosis with polyangiitis (EGPA; also known as Churg–Strauss syndrome) is a rare disease characterized by allergic granulomatosis and necrotizing vasculitis after peripheral and tissue eosinophilia . The mortality and prognosis of EGPA are related to disease severity, as assessed by five-factor scores (FFSs) , and the survival rate at 5 years after myocardial involvement is reported to be one third of that without myocardial involvement . The mainstay of treatment for EGPA is systemic corticosteroid therapy; some patients receive additional treatment with immunosuppressive agents, such as cyclophosphamide and azathioprine . However, combined therapy with corticosteroids and cyclophosphamide afforded little benefit in some EGPA patients with mononeuritis multiplex, heart failure, or systemic vasculitis associated with antineutrophil cytoplasmic autoantibody [5, 6]. We previously showed that intravenous immunoglobulin (IVIG) therapy was effective against severe mononeuritis multiplex or heart failure in patients with EGPA that did not respond to corticosteroid–cyclophosphamide treatment .
Regulatory T (Treg) cells play a key role in balancing immune responses and in maintaining peripheral tolerance against antigens and allergens . We previously reported a lower frequency of Treg cells at the onset of disease in patients with EGPA than in patients with asthma and that the frequency of Treg cells increased at remission of EGPA . Recent reports show that IVIG regulates the activation of CD4+CD25+ Treg cells in autoimmune disease . Possible mechanisms involved in the IVIG-mediated modulation of Treg functions include the activation and induction of Treg cells by the Fc region of IVIG and enhancement of Treg function through increased expression of forkhead box P3 (FOXP 3), transforming growth factor-β, interleukin (IL)-10, and cytotoxic T-lymphocyte antigen 4 (CTLA-4) [11–13]; direct interaction of self-reactive natural autoantibodies with T-cell surface molecules [14, 15]; and the suppression of allogeneic T-cell responses through direct activation of Treg cells . We found that prolonged IVIG treatment increased the frequencies of Treg cells, defined as the CD25+ subpopulation among CD4+ T cells producing IL-10 and the FOXP3+ subpopulation among CD4+ T cells . Several authors have reported that IVIG treatment may reduce the amount of corticosteroids needed for maintenance of remission of EGPA [17, 18]. However, whether the clinical and immunologic efficacy of IVIG is affected by the timing of administration (e.g., during initial treatment of EGPA or at relapse after remission) is unknown. In addition, the characteristics of patients’ disease condition that influence whether remission is achieved after single or multiple courses of IVIG have not previously been assessed. Here we evaluated whether the frequency of Treg cells differed depending on when IVIG was provided relative to the start of conventional therapy for EGPA.
This study was a retrospective analysis for which we recruited patients given IVIG during initial treatment or at relapse after remission, to investigate the clinical and immunologic efficacy of IVIG therapy for EGPA patients.
Between March 2005 and November 2011, 17 patients with EGPA were diagnosed according to the classification criteria of the American College of Rheumatology at our hospital . Two of these 17 patients were enrolled in the study reported in reference number 17; the remaining 15 patients were new patients. Multiple mononeuritis, a type of motor nerve dysfunction, was evaluated by using the manual muscle test (MMT); responses were scored (from 0 to 5) on the Medical Research Council scale, and sensory nerve dysfunction was evaluated through clinical examination. Lung involvement was considered to be present when any consolidation, ground-glass opacity, nodules within such opacity, interlobular septal thickening, bronchial wall thickening, lymph node enlargement, pleural effusion evident upon high-resolution computer tomography, or eosinophilic infiltration detected by lung biopsy was present. The heart was considered to be involved when any chest pain, chest discomfort, back pain, palpitations, echocardiographic abnormality, Holter electrocardiographic abnormality, elevation of B-type natriuretic peptide level, or [123I]-meta-iodobenzylguanidine myocardial imaging abnormality was evident . Gastrointestinal involvement was indicated by the presence of symptoms of upper or lower abdominal pain, diarrhea, constipation, or positive endoscopic signs combined with confirmation of eosinophilic infiltration by biopsy. Skin involvement was defined as the presence of purpura, erythema, livedo, ulceration, or acrocyanosis when a nodule, accompanied by eosinophilic infiltration, was detected by biopsy. Central nervous system involvement was defined as the presence of headache, visual disorder, abnormal visual sensation, cerebral infarction, bleeding, or cranial nerve dysfunction. Renal involvement was defined by any presence of eosinophils in urine, glomerular nephritis, nephrosis, renal dysfunction (i.e., the creatinine level was 20% or more higher than the baseline figure), or proteinuria (>0.5 g/dL). Disease severity in all EGPA patients was evaluated by using FFS 2009 .
All patients treated with IVIG had severe neuropathy (MMT score, <3) or cardiac involvement, including severe heart failure, myocarditis, or pericarditis, which did not improve or achieve remission after at least one month of treatment with conventional therapy (corticosteroids, immunosuppressants, or both; the initial dose of corticosteroid was approximately 1 mg/kg prednisolone daily for at least 1 month) . Among our 17 patients with EPGA, 10 received IVIG during initial treatment with conventional therapy; the remaining 7 patients achieved first remission after conventional treatment without IVIG and therefore received their first IVIG on relapse after remission. Remission was defined as the absence of any clinical signs or symptoms of active vasculitis for at least 6 months. Relapse was defined as the presence of active disease combined with the recurrence, after initial remission, of vasculitis symptoms (with or without an increase in the percentage of eosinophils among white blood cells [WBCs]). Patients who relapsed required resumption of immunosuppressive therapy or increased doses of immunosuppressant.
Before treatment with IVIG (Venilon, Teijin, Tokyo, Japan; 400 mg/kg daily for 5 days), all patients received corticosteroids (initial dose, about 1 mg/kg prednisolone daily for at least 1 month) with or without immunosuppressants. Nine patients with treatment-refractory mononeuritis multiplex or heart failure received additional courses of IVIG at 2 to 12 months after the initial IVIG therapy until they achieved remission. As in our previous study , we measured the percentage of CD25+CD4+ Treg cells (that is, CD25+ cells among CD4+ Treg cells) and FOXP3+CD4+ T cells (that is, FOXP3+ cells among CD4+ T cells), which we defined as Treg cells, in the peripheral blood of all patients before and at 1 month after the initial IVIG treatment; the ratio between these values was used to represent the IVIG-associated change in the proportion of FOXP3+CD4+ T cells. The ethics committee of our hospital approved the study, and we obtained informed consent from each patient.
The number of eosinophils in whole blood before and at the onset of IVIG therapy, and the serum IgG concentration before IVIG, were measured in all patients. Eosinophils and WBCs in the peripheral blood were counted by hemocytometry. The frequencies of CD25+CD4+ T cells and of FOXP3+CD4+ T cells were determined as described by Abdulahad et al. . PE-conjugated anti-human FOXP3 and Peridinin chlorophyll conjugates of mouse IgG1 and an anti-CD4 antibody were purchased from BD Biosciences (Rikaken Co. Ltd., Tokyo, Japan). In brief, whole-blood lymphocytes were incubated with FITC-conjugated anti-CD4, and FOXP3+CD4+ T cells (defined as FOXP3+ cells among CD4+ T cells) were identified by incubating whole-blood lymphocytes with PE-conjugated anti-FOXP3 antibody after cell permeabilization with 4% (v/v) formaldehyde and 0.1% (w/v) saponin. The expression of surface and intracellular markers by CD4+ T cells was analyzed by means of flow cytometry (FACSCalibur, Nippon Becton Dickinson, Tokyo, Japan).
All values are expressed as mean ± 1 SD unless otherwise specified. Statistical comparisons among groups were achieved by using two-way analysis of variance (ANOVA) according to a repeated-measures algorithm, followed by post-hoc comparisons by using the Newman–Keuls test. The two mean values obtained by this process were compared by using the Wilcoxon matched-pairs t test. Correlation coefficients were obtained by using Spearman’s rank correlation test. P values less than 0.05 were considered statistically significant. Statistical analysis was performed by using SPSS for Windows, version 20 (SPSS Inc., Chicago, IL).
A single course of IVIG after conventional corticosteroid-based therapy was sufficient to achieve remission of EGPA in 3 of the 10 patients who received IVIG during their initial treatment and in 5 of the 7 patients treated with IVIG after relapse of EGPA. The other nine patients required 3.6 ± 1.6 course of IVIG before remission was achieved. Overall, 8 patients that received a single round of IVIG treatment during initial therapy to achieve remission, and the remaining 9 patients needed two or more IVIG treatments after relapse to achieve a second remission. Although the dosage of prednisolone at initial treatment did not differ between the two groups, the proportion of patients who received immunosuppressants was greater (P < 0.05) for those given IVIG during initial therapy than for those treated with IVIG after relapse of EGPA (Table 1). The dosage of prednisolone at initial IVIG in patients with relapsed disease was lower (P < 0.05) than that for patients under initial treatment for EGPA. Whereas 9 of the 10 patients (90.0%) who received IVIG during their initial treatment received immunosuppressants in addition to corticosteroids, only 3 of the 7 patients (42.9%) with relapsed EGPA received immunosuppressants during their initial treatment (P < 0.05) (Table 1).
The number of eosinophils in peripheral blood at initial IVIG was higher (P < 0.05) and the percentage of FOXP3+CD4+ T cells was lower (P < 0.05) in patients with relapsed EGPA than in patients undergoing initial treatment (Table 1). The minimal MMT score at onset of treatment was lower (P = 0.05) in patients undergoing initial treatment than in patients treated for relapsed EGPA. The percentage of patients with myocardial involvement at initial diagnosis of EGPA was higher (P = 0.05) in those treated because of relapsed disease than in those who received their initial treatment for EGPA (Table 2). The diagnoses according to the criteria of the American College of Rheumatology are shown in Table 3.
The percentage of FOXP3+CD4+ T cells at initial IVIG was significantly (P < 0.05) greater in the 8 patients who achieved remission after a single round of IVIG compared with the 9 patients who needed two or more IVIG treatments for remission of EGPA (Figure 1). In all patients, the dosage of prednisolone at initial IVIG was significantly statistically correlated (P < 0.05, rs = 0.57) with percentage of FOXP3+CD4+ T cells (Figure 2a) and significantly inversely correlated (P < 0.05, rs = −0.53) with the ratio between the number of FOXP3+CD4+ T cells before to that at 1 month after the initial IVIG treatment (Figure 2b). The percentage of FOXP3+CD4+ T cells before the initial course of IVIG was greater than that at 1 month afterward in 5 of the 9 patients who needed two or more rounds of IVIG to achieve remission of EGPA (Figure 2b). In all patients, serum IgG concentration (IU/mL) before initial IVIG was significantly inversely correlated (P < 0.01, rs = −0.62) with the dosage of prednisolone at initial IVIG (Figure 3) but was not correlated with duration or the total dose of prednisolone taken during this study. The number of eosinophils in peripheral blood before IVIG was significantly inversely correlated (P < 0.05, rs = −0.56) with the percentage of FOXP3+CD4+ T cells (Figure 4a) but not with the ratio of FOXP3+CD4+ T cells before to that 1 month after initial IVIG (Figure 4b).
Treatment with IVIG has shown efficacy in autoimmune and immune-mediated inflammatory diseases [14, 15, 22], including microscopic polyangiitis, granulomatosis with polyangiitis (that is, Wegener’s granulomatosis), and antineutrophil cytoplasmic antibody-associated systemic vasculitis [5, 23, 24]. The phase at which IVIG is most effective in EGPA patients with neuropathy or cardiac failure is unknown, as are the optimal number and timing of courses of IVIG to induce remission. Among patients with systemic lupus erythematosis, responders and nonresponders to IVIG treatment differed in their serum concentrations of C4, SS-A, and SS-B . We previously reported that the number of courses of IVIG needed to induce remission was 2.6 ± 2.1 times greater to achieve first remission . Whether differences in disease characteristics affect the need for one compared with multiple rounds of IVIG treatment to induce remission is unknown. We here show that patients with severe EGPA that receive IVIG during their initial treatment with high-dose systemic corticosteroids likely need multiple courses of IVIG to achieve remission. In addition, an increased percentage of FOXP3+CD4+ T cells before IVIG treatment may indicate an increased likelihood of induction of remission of EGPA. In patients receiving their initial treatment for EGPA, the percentage of FOXP3+CD4+ T cells was increased after conventional treatment comprising corticosteroids with or without immunosuppressants. However, the percentage of FOXP3+CD4+ T cells at one month after IVIG failed to increase in more than half of the patients given prednisolone doses exceeding 40 mg during IVIG treatment (Figure 2b), and high-dose prednisolone may suppress the serum IgG level (Figure 3). We consider that many inflammatory mediators are present in various quantities in serum during the acute disease phase associated with initial treatment of EGPA; these mediators might therefore out-compete IVIG and suppress the differentiation of Treg (FOXP3+CD4+) cells.
The number of Treg cells (i.e., FOXP3+CD4+ T cells) in patients with severe EGPA did not increase rapidly after IVIG, and this cell population was increased in patients requiring multiple courses of IVIG to achieve clinical remission (data not shown). We previously reported that the frequency of CD25+CD4+ T cells correlated with FOXP3+CD4+ Treg cells in EGPA patients with severe mononeuritis multiplex or cardiac dysfunction and remained greater than that in EGPA patients without IVIG treatment for as long as 2 years after completion of IVIG therapy .
EGPA manifests as necrotizing vasculitis and eosinophilic tissue inflammation [25, 26]. In both of our current patient groups, the number of eosinophils in peripheral blood decreased after conventional treatment and was significantly inversely correlated with the percentage of FOXP3+CD4+ T cells. However, the number of eosinophils at initiation of IVIG treatment was unrelated to any increase in the percentage of FOXP3+CD4+ T cells after IVIG. These results indicate that disease activity and response to IVIG likely are reflected not only in the number of peripheral eosinophils but also in levels of Th2 cytokines [27–29], Th17 [27, 28, 30, 31], or other unknown mediators. Other possible mechanisms involved in IVIG-mediated inhibition are the differentiation and function of Th17 cells , the upregulation of Treg cells by induction of cyclooxygenase-2-dependent prostaglandin E2 , or the suppression of inflammation by upregulating FcγRIIB on macrophages via IL-4 produced by basophils . Although in vitro experiments have hinted at many novel findings regarding the mechanisms of IVIG, it is necessary to verify the clinical or immunologic effects of IVIG in vivo. It is useful clinically to determine optimal dose level, timing of initial dose, number of courses, and interdose interval for maximally effective IVIG therapy.
We conclude that most patients with otherwise uncomplicated non-severe EGPA (i.e., MMT score greater than 3, lack of cardiac involvement) who receive IVIG at relapse can achieve remission after a single round of IVIG therapy. However, many patients with severe EGPA (i.e., MMT score less than 3, cardiac abnormalities) who are undergoing initial treatment with conventional therapy including high-dose prednisolone will require multiple courses of IVIG to achieve remission of EGPA.
Cytotoxic T-lymphocyte antigen 4
Eosinophilic granulomatosis with polyangiitis
Forkhead box P3
- Treg cells:
Regulatory T cells
Manual muscle test
Myeloperoxidase-specific antineutrophil cytoplasmic autoantibody
White blood cells.
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All authors declare that they have no competing interests.
NT examined the patients, analyzed data and statistics, was the main contributor to manuscript preparation, and was involved in manuscript preparation and editing. CO examined the patients and contributed to discussions about the patients. HS assayed FOXP3+CD4+ Treg cells in peripheral blood of patients. TT and KA contributed to discussions about the manuscript. All authors read and approved the final manuscript.
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Tsurikisawa, N., Saito, H., Oshikata, C. et al. High-dose intravenous immunoglobulin therapy for eosinophilic granulomatosis with polyangiitis. Clin Transl Allergy 4, 38 (2014). https://doi.org/10.1186/2045-7022-4-38
- Eosinophilic granulomatosis with polyangiitis
- Churg–Strauss syndrome
- Intravenous immunoglobulin
- Regulatory T cells