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  • Poster presentation
  • Open Access

Oleosines in sesame allergy

  • 1,
  • 1,
  • 1 and
  • 2
Clinical and Translational Allergy20133 (Suppl 3) :P170

https://doi.org/10.1186/2045-7022-3-S3-P170

  • Published:

Keywords

  • System Extract
  • Skin Prick Testing
  • Anaphylactic Reaction
  • Sesame Seed
  • Commercial Extract

Background

Although infrequent, sesame allergy is well known. Most of the cases are due to sensitization to water-soluble proteins. However, lipophilic proteins may be important [1].

Methods

We present the case of a 31 y.o. woman with repeated anaphylactic reactions most of them upon eating at oriental restaurants. A vast allergic study was carried out in another center ruling out allergy/intolerance to several food additives and foods. The key fact for the diagnosis was an anaphylactic reaction upon eating home-made humus made of chickpea, olive oil and sesame oil. We performed wide skin prick testing (SPT) and specific IgE (both CAP and ISAC) for nuts, legumes, and sesame seed (commercial extract, natural raw sesame seed) as well as prick test, prick-prick, and prick patch with sesame oil, and open food challenge test (OFCT) with sesame seed.

Results

SPT with foods including sesame seed: negative. Skin prick-patch test with sesame oil: positive. SDS-PAGE/immunoblotting: no IgE fixation against water soluble sesame extract proteins; IgE fixation to a 17-kDa protein from the lipidic sesame extract. This protein was identified as an oleosin (Ses i 4). OFCT positive with 2 grams of sesame seeds.

Conclusion

Oleosins have been described by others [2], and are important allergens from sesame seed. Since they are hydrophobic, they are not present at commercial extracts or extract prepared from sesame seed in saline, or the CAP system extract. ISAC only contains Ses i 1. Prick test has to be performed with prick-patch test with immediate reading (15-20 min) [3]. The lipidic part of the seed has to be considered when studying unexplained cases of suspected sesame allergy.

Disclosure of interest

None declared.

Authors’ Affiliations

(1)
Allergy, Clínica de Asma y Alergia Dres. Ojeda, Madrid, Spain
(2)
Research, Diater Laboratories, Madrid, Spain

References

  1. Gangur V, Kelly C, Navuluri L: Sesame allergy: a growing food allergy of global proportions?. Ann Allergy Asthma Immunol. 2005, 95 (1): 4-11. 10.1016/S1081-1206(10)61181-7.View ArticlePubMedGoogle Scholar
  2. Leduc V: Identification of oleosins as major allergens in sesame seed allergic patients. Allergy. 2006, 61: 349-356. 10.1111/j.1398-9995.2006.01013.x.View ArticlePubMedGoogle Scholar
  3. Alonzi C: Diagnosing IgE-mediated hypersensitivity to sesame by an immediate-reading “contact test” with sesame oil. J Allergy Clin Immunol. 2011, 127: 1627-9. 10.1016/j.jaci.2011.01.050.View ArticlePubMedGoogle Scholar

Copyright

© Ojeda et al; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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