Epigenome-wide association study identifies DNA methylation markers for asthma remission in whole blood and nasal epithelium

Table 3 Top ten CpG sites in meta-analysis of ComR in relation to methylation in whole blood showing the same direction of effect in discovery and replication cohort

CpG	CHR	Great annotation (distance to TSS)	Discovery				Replication-EGEA				Meta-analysis
CpG	CHR	Great annotation (distance to TSS)	Coef	SE	P	N	Coef	SE	P	N^b	P.meta	Direction
cg24788483^a	10	HABP2 (− 401132), TCF7L2 (+ 201,644)	− 1.31E−02	2.11E−03	4.62E−10	54	− 1.348	0.544	0.013	56 (53/3)	8.49E−10	–
cg15223066	10	ITGB1 (− 23,896), NRP1 (+ 354472)	− 8.56E−03	1.39E−03	6.65E−10	54	− 0.658	0.470	0.161	92 (89/3)	1.13E−06	–
cg26909813	17	RPRML (-605)	− 2.68E−02	4.15E−03	1.06E−10	54	− 0.621	0.539	0.249	95 (92/3)	1.52E−06	–
cg02341571	15	PATL2 (+ 150)	− 1.69E−02	2.71E−03	4.39E−10	54	− 0.253	0.388	0.515	64 (62/2)	2.59E−06	–
cg00269245	16	SLC12A3 (− 41116), NUP93 (+ 93986)	1.13E−02	1.86E−03	1.14E−09	54	0.832	1.109	0.453	70 (68/2)	4.62E−06	+ +
cg24833566	4	MTNR1A (− 152680), FAT1 (+ 15608)	1.02E−02	1.71E−03	2.38E−09	54	0.658	0.758	0.386	77 (74/3)	6.87E−06	+ +
cg25881850	11	KCNK4 (− 1426)	− 1.23E−02	2.23E−03	3.43E−08	54	− 0.838	0.816	0.304	68 (67/1)	9.09E−06	–
cg24730612	14	FOS (− 95503), TMED10 (− 6640)	− 1.47E−02	2.50E−03	4.15E−09	54	− 0.260	0.489	0.595	62 (61/1)	1.09E−05	–
cg23250019	11	SLC29A2 (+ 818)	2.15E−02	4.00E−03	7.62E−08	54	0.645	0.541	0.234	82 (79/3)	1.61E−05	+ +

P.meta: the P value of meta-analysis of results from discovery and EGEA by using weighted Z-scores method
Direction: the direction of regression coefficient in discovery and EGEA
A list of all 129 significant CpG sites identified in discovery cohort and the information of replication in EGEA is shown in Additional file 2: Table S9
^aThe CpG site was replicated with meta P value < 1.14 × 10^–7
^bN (persA/ComR), N varies from one CpG to another because of the methylC-capture sequencing method used and different QC criteria in EGEA

ISSN: 2045-7022