Table 3 Methods addressing several MIE’s and KE’s shown in Fig. 1

MIE 1, 2, 3 & KE1

M cells

Allergen quantification (SDS-PAGE, Western blot and microscopy)

Integrity of ZO-1 (microscopy).

T84

Monolayer integrity (TEER) Cytokine production (ELISA)

HCT-8

Monolayer integrity (TEER) Cytokine production (ELISA)

Caco-2

Monolayer integrity (TEER and Lucifer Yellow)

Allergen quantification (ELISA, SDS-Page, Western blot and LC–MS,)

Integrity of A20 (Western blot and RT-qPCR blot and microscopy)

Allergen transport (RBL activation test)

Gene expression (RT-qPCR)

HT-29

Monolayer integrity (TEER).

Allergen quantification (ELISA)

Integrity of A20 (Western blot and RT-qPCR

KE 2 & 3

Mouse BM-DCs

Allergen uptake (flow cytometry)

Migration assay (flow cytometry)

Cytokine production (ELISA)

DCs maturation (flow cytometry)

THP-1-derived DCs

Allergen uptake (flow cytometry)

Gene expression (RT-qPCR)

Cytokine production (ELISA)

Human Mo-DCs

Expression of DC markers (flow cytometry)

KE 4

Human T cell clones

T cell proliferation ([³H]-thymidine)

Cytokine production (ELISA)

T cell activation (flow cytometry)

Human peripheral blood mononuclear cells (PBMCs)

T cell proliferation (CFSE or [³H]-thymidine)

Cytokine production (flow cytometry)

T cell activation (flow cytometry)

Gene expression (RT-qPCR)

Expression of T cell markers (flow cytometry)

Mouse MLN-isolated T cells

Cytokine production (ELISA)

Mouse LP-isolated mononuclear cells

T cell proliferation (CFSE)

Cytokine production (flow cytometry)

KE 2, 3 & 4

Co-culture: BM-DCs/primed T cells

Allergen uptake (flow cytometry)

T cell cytokine production (ELISA)

DCs maturation (flow cytometry)