Fig. 3

Quantity and specific gene expression of Tregs showed up-regulation in the farming group after LPS and PPG stimulation. a CD4 + CD25 + FOXP3 + T cell percentage in CBMCs (relative to unstimulation). The values are represented as mean ± SEM. T-test was used to compare the two groups. b FOXP3 gene expression in neonatal CBMCs. c GITR gene expression in neonatal CBMCs. d CTLA4 gene expression in neonatal CBMCs. e TLR2 gene expression in neonatal CBMCs. We used fold difference (fd) = 2^(− delta delta CT) to present the gene expression. The gating strategy used is illustrated in Additional file 1: S1. The values are represented as median (25%/75% IQR), and Mann–Whitney Rank Sum Test was used to compare the two groups. Statistical significance is indicated by P values < 0.05