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Fig. 6 | Clinical and Translational Allergy

Fig. 6

From: Protease resistance of food proteins: a mixed picture for predicting allergenicity but a useful tool for assessing exposure

Fig. 6

Selected SDS-PAGE and immunoblot samples are shown for carp parvalbumin, Cyp c 1. A At pH 2.5 Cyp c 1 is readily digested by pepsin. With decreasing PPR, presence of residual breakdown peptide increases, but these are not recognized on immunoblot. Pepsin band on SDS-PAGE is boxed in red. B Cyp c 1 is highly resistant to pancreatin with some appearance of similar breakdown peptides as observed upon exposure to pepsin. C IgE immunoblotting confirms pepsin and pancreatin resistance characteristics observed wit rabbit IgG

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Clinical and Translational Allergy

ISSN: 2045-7022

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