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Fig. 3 | Clinical and Translational Allergy

Fig. 3

From: Protease resistance of food proteins: a mixed picture for predicting allergenicity but a useful tool for assessing exposure

Fig. 3

Selected SDS-PAGE and immunoblot samples are shown for both albumins, Ara h 2 and pea PA2 albumin. A Ara h 2 displays complete resistance up to 1 h to pepsin at acidic pH, whereas the pea albumin is undetectable after 5 min. Both albumins are unaffected by pepsin at pH 4.0. B Pea albumin PA2 appears to be more resistant to pancreatin than Ara h 2. Surprisingly the disappearance of Ara h 2 is transient when it has not been exposed to acidic pH and active pepsin. C High resistance of Ara h 2 to pepsin is confirmed for IgE. For pea albumin susceptibility could not be confirmed because no sera with pea albumin reactive IgE were found. Susceptibility characteristics of Ara h 2 to pancreatin were also confirmed with IgE

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Clinical and Translational Allergy

ISSN: 2045-7022

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