Table 2 Methods for identifying mite allergens in the confirmation phase
From: Consideration of methods for identifying mite allergens
Authors | Protein type | Expression system | Method of isolation | % Patients with IgE binding | Test for allerginicity (% Positive) | PubMed ID | Allergen | Year |
|---|---|---|---|---|---|---|---|---|
Ferrandiz et al. [18] | N |  | Affinity chromatography (mAB) | 80–91 | NA | 8556562 | Der s1 Der s2 | 1995 |
King et al. [6] | N | Â | Chromatography (protease activity) | 92 | NA | 8876548 | Der p 9 | 1996 |
Fujikawa et al. [25] | N | Â | Affinity chromatography (AB) | Â | Â | Â | Der f 14 | 1996 |
Wey et al. [53] | N | Â | Affinity chromatography (mAB) | 37.50 | Skin prick (45%) | 10592827 | Der p 94 kD | 1997 |
N | Â | Affinity chromatography (mAB) | >Â 80 | NA | 9723675 | Der f 11 Der p 11 | 1998 | |
Olsson et al. [49] | N and R | E. coli and Baculovirus | Affinity chromatography (His-tagged) | Â | Basophil activation | 9756203 | Lep d 2 | 1998 |
Kawamoto et al. [5] | R | E. coli | Affinity chromatography (GST-tagged) | 39 | Basophil activation | 10381565 | Der f 6 | 1999 |
Binder et al. [44] | R | E. coli | Affinity chromatography (His-tagged) | 25 | Basophil activation skin prick | 11673567 | Plo i 1 (group 20 homologue) | 2001 |
Cheong et al. [48] | R | E. coli | Affinity chromatography (GST-tagged) | 50 | Skin prick | 12708986 | Blo t 3 | 2003 |
Ramos et al. [52] | N | Â | Affinity chromatography (mAB) | 63 | NA | 15080814 | Blo t 1 | 2004 |
Cai et al. [65] | R | E. coli | Affinity chromatography (His-tagged) | Â | NA | 17639694 | Der f 3 | 2007 |
Gao et al. [63] | R | E. coli | Affinity chromatography (His-tagged) | 58.00 | Skin prick | Â | Blo t 21 | 2007 |
Weghofer et al. [29] | R | E. coli | Ion exchange chromatography (no tag) | 26 | Basophil activation | 18445190 | Der p 21 | 2008 |
Weghofer et al. [66] | R | E. coli | Ion exchange chromatography (no tag) | 31 | Basophil activation | 18520154 | Der p 5 | 2008 |
Beckham et al. [33] | R | E. coli and P. pastoris | Affinity chromatography (His-tagged) ion exchange chromatography | NA | NA | 19812030 | Sar s 3 | 2009 |
Cui et al. [67] | R | E. coli | Affinity chromatography (His-tagged) | NA | NA | 19951588 | Der f 3 | 2009 |
Cui et al. [68] | R | E. coli | Affinity chromatography (His-tagged) | NA | NA | 20939383 | Der f 7 | 2010 |
Bordas-Le Floch et al. [50] | N and R | E. coli. and P. pastoris | Ion exchange chromatography (no tag) | NA | Basophil activation and mouse model | 22286395 | Der p 2 | 2012 |
Weghofer et al. [30] | R | E. coli | Hydrophobic interaction and ion exchange chromatography (no tag) | 74 | Basophil activation | 23460742 | Der p 23 | 2013 |
An et al. [27] | N |  | Chromatography (gel filtration and ion exchange) | 63–86 | Skin prick (60–70%) and basophil activation | 23481662 | Der f 25 Der f 28–30 | 2013 |
Banerjee et al. [4] | N and R | E. coli | Affinity chromatography (His-tagged or AB) | 5–67 | NA |  | Der p 11 | 2015 |
Lin et al. [69] | R | E. coli | Affinity chromatography (His-tagged) | NA | Skin prick (42.1%) and mouse model | 26623108 | Der f 27 | 2015 |
Chan et al. [32] | R | E. coli | Affinity chromatography (His-tagged) | 100 | Skin prick (50%) | 25445830 | Der f 24 | 2015 |
Cui et al. [70] | R | E. coli | Affinity chromatography (His-tagged) | 41 | NA | 26842967 | Der f 4 | 2016 |
Lin et al. [35] | R | E. coli | Affinity chromatography (His-tagged) | NA | Skin prick (24.3%) | 27158348 | Der f 29b | 2016 |