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Table 2 Methods for identifying mite allergens in the confirmation phase

From: Consideration of methods for identifying mite allergens

Authors Protein type Expression system Method of isolation % Patients with IgE binding Test for allerginicity (% Positive) PubMed ID Allergen Year
Ferrandiz et al. [18] N   Affinity chromatography (mAB) 80–91 NA 8556562 Der s1 Der s2 1995
King et al. [6] N   Chromatography (protease activity) 92 NA 8876548 Der p 9 1996
Fujikawa et al. [25] N   Affinity chromatography (AB)     Der f 14 1996
Wey et al. [53] N   Affinity chromatography (mAB) 37.50 Skin prick (45%) 10592827 Der p 94 kD 1997
Tsai et al. [61, 64] N   Affinity chromatography (mAB) > 80 NA 9723675 Der f 11 Der p 11 1998
Olsson et al. [49] N and R E. coli and Baculovirus Affinity chromatography (His-tagged)   Basophil activation 9756203 Lep d 2 1998
Kawamoto et al. [5] R E. coli Affinity chromatography (GST-tagged) 39 Basophil activation 10381565 Der f 6 1999
Binder et al. [44] R E. coli Affinity chromatography (His-tagged) 25 Basophil activation skin prick 11673567 Plo i 1 (group 20 homologue) 2001
Cheong et al. [48] R E. coli Affinity chromatography (GST-tagged) 50 Skin prick 12708986 Blo t 3 2003
Ramos et al. [52] N   Affinity chromatography (mAB) 63 NA 15080814 Blo t 1 2004
Cai et al. [65] R E. coli Affinity chromatography (His-tagged)   NA 17639694 Der f 3 2007
Gao et al. [63] R E. coli Affinity chromatography (His-tagged) 58.00 Skin prick   Blo t 21 2007
Weghofer et al. [29] R E. coli Ion exchange chromatography (no tag) 26 Basophil activation 18445190 Der p 21 2008
Weghofer et al. [66] R E. coli Ion exchange chromatography (no tag) 31 Basophil activation 18520154 Der p 5 2008
Beckham et al. [33] R E. coli and P. pastoris Affinity chromatography (His-tagged) ion exchange chromatography NA NA 19812030 Sar s 3 2009
Cui et al. [67] R E. coli Affinity chromatography (His-tagged) NA NA 19951588 Der f 3 2009
Cui et al. [68] R E. coli Affinity chromatography (His-tagged) NA NA 20939383 Der f 7 2010
Bordas-Le Floch et al. [50] N and R E. coli. and P. pastoris Ion exchange chromatography (no tag) NA Basophil activation and mouse model 22286395 Der p 2 2012
Weghofer et al. [30] R E. coli Hydrophobic interaction and ion exchange chromatography (no tag) 74 Basophil activation 23460742 Der p 23 2013
An et al. [27] N   Chromatography (gel filtration and ion exchange) 63–86 Skin prick (60–70%) and basophil activation 23481662 Der f 25 Der f 28–30 2013
Banerjee et al. [4] N and R E. coli Affinity chromatography (His-tagged or AB) 5–67 NA   Der p 11 2015
Lin et al. [69] R E. coli Affinity chromatography (His-tagged) NA Skin prick (42.1%) and mouse model 26623108 Der f 27 2015
Chan et al. [32] R E. coli Affinity chromatography (His-tagged) 100 Skin prick (50%) 25445830 Der f 24 2015
Cui et al. [70] R E. coli Affinity chromatography (His-tagged) 41 NA 26842967 Der f 4 2016
Lin et al. [35] R E. coli Affinity chromatography (His-tagged) NA Skin prick (24.3%) 27158348 Der f 29b 2016
  1. R: recombinant. N: native. NA: not attempted or too few patients to draw conclusions about prevalence