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Table 2 Preparation of the DBPCFC verum dilution series

From: Development and validation of a standardized double-blind, placebo-controlled food challenge matrix for raw hazelnuts

A. Dilution series of the hazelnuts suspended in water to develop the verum series

B. Dilution series of the hazelnut DBPCFC verum series

Instructions for preparation/portion

Additional information

Portion number

Dilution number (65 g)

Powder mix I (g)

Served portion size (g)

Dilution factor of served hazelnuts

Hazelnut (mg)/portion

Hazelnut proteins (mg)/portion

Ppm (mg/kg) hazelnuts

1

0

35.0

2.50

x

0

0

0

2

4

35.0

2.50

1/10

0.0370

0.005

14.8

3

3

35.0

2.50

1/10

0.3704

0.054

148.0

4

2

35.0

2.50

1/10

3.691

0.535

1476.9

Instructions for preparation/portion

Additional information

Portion number

Dilution number (65 g)

Powder mix II (g)

Served portion size (g)

Dilution factor of served hazelnuts

Hazelnut (g)/portion

Hazelnut proteins (mg)/portion

Ppm (mg/kg) hazelnuts

5

1

40.2

1.34

1/3

0.04

5.37

27,721.3

6

1

40.2

4.01

1/3

0.11

16.11

27,721.3

7

1

40.2

12.02

1/3

0.33

48.33

27,721.3

8

1

40.2

36.07

1/3

1.00

145.00

27,721.3

9

1

40.2

108.22

1

3.00

435.00

27,721.3