Immune response induced after intra-nasal administrations of BLG or whole milk. Mice received 10 μl of PBS, purified BLG or milk intranasally (n = 20/group). (A) Serum samples were collected after 6 intra-nasal administrations and BLG-specific IgG1 were quantified on individual serum samples. The numbers of positive mice are indicated above each group. A response was considered significant when higher than NSB + 3σ. (B) Systemic specific IL-13, IL-5 and IL-4 secretion was assessed after ex vivo restimulation of splenocytes with BLG.