Volume 5 Supplement 4

Abstracts from the 10th Symposium of Experimental Rhinology and Immunology of the Nose (SERIN 2015)

Open Access

Immunologic responses to the major allergen of Olea Europaea in local and systemic allergic rhinitis subjects

  • Paloma Campo1,
  • Carmen Rondon2,
  • Mayte Villalba3,
  • Esther Barrionuevo2,
  • Luisa Galindo2,
  • Juan Carlos Lopez-Rodriguez3,
  • Maria Jose Torres2,
  • Cristobalina Mayorga2 and
  • Miguel Blanca2
Clinical and Translational Allergy20155(Suppl 4):P19

https://doi.org/10.1186/2045-7022-5-S4-P19

Published: 26 June 2015

Background

Ole e 1 is one of the major allergens from olive tree pollen. Up to date there are no specific studies that evaluate in depth the in vitro responses to this purified allergen. The goal of the study was to thoroughly evaluate the celullar responses to nOle e 1 in allergic rhinitis (AR) and local allergic rhinitis (LAR) patients with sensitization to olive tree pollen (OL) demonstrated by nasal allergen provocation test (NAPT).

Methods

Twelve subjects with AR (+NAPT with OL, + skin testing and specific IgE (sIgE) to OL), 12 subjects with LAR (+ NAPT with OL, - skin testing and sIgE to OL), and 12 subjects as control group (CG) (- NAPT, - skin testing and sIgE to OL) were selected. Basophil activation tests (BAT) with OL and nOle e 1, along with dendritic cell (DC) maturation/proliferation studies in response to nOle e 1 stimulation, were carried out in all subjects. Local ethical committee approved the study.

Results

All AR subjects had positive BAT responses to OL and 10/12 to nOle e1 (83%); 8/12 LAR (66.6%) had a positive BAT with OL and 4/12 (33%) to nOle e1, with only one subject of the control group with a positive BAT to both OL and nOle e1 (8%). DC proliferation and maturation were increased in SAR>LAR>CG but with no significant differences (maturation: 66.7%/57%/50%; proliferation: 40%/20%/0%).

Conclusion

BAT with OL and nOle e1 in LAR group showed sensitivity between 66.6 and 33%, demonstrating specific basophil activation with pollens in patients with LAR. DC proliferation and maturation were demonstrated in SAR and LAR subjects although with no significant differences with CG.

Authors’ Affiliations

(1)
Regional Hospital of Malaga
(2)
Regional Hospital of Malaga, UGC Allergy
(3)
Department of Biochemistry and Molecular Biology, Faculty of Chemistry, Complutense University of Madrid

Copyright

© Campo et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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