Volume 5 Supplement 4

Abstracts from the 10th Symposium of Experimental Rhinology and Immunology of the Nose (SERIN 2015)

Open Access

Thymic Stromal Lymphopoietin (TSLP) promotes human eosinophil-basophil in situ hemopoieisis, and its secretion from human nasal epithelium is a function of TSLP genotype

  • Judah Denburg1,
  • Claudia Hui1,
  • Helen Neighbour1,
  • Delia Heroux1,
  • Loubna Akhabir2 and
  • Andrew Sandford2
Clinical and Translational Allergy20155(Suppl 4):O3

https://doi.org/10.1186/2045-7022-5-S4-O3

Published: 26 June 2015

Background

Allergic diseases are characterized by tissue eosinophilic and basophilic inflammation. There is substantial evidence that this particular inflammatory profile results from the migration to tissues of a common eosinophil-basophil (Eo/B) progenitor that undergoes a differentiative process regulated by local cytokines, termed in situ hemopoiesis. We therefore investigated the role and mechanisms of TSLP involvement in human Eo/B in situ hemopoiesis in relation to atopy. Also, since candidate gene and genome-wide association studies have identified ‘protective’ associations between the single nucleotide polymorphism (SNP) rs1837253 in the TSLP gene and risk for allergy, asthma and airway hyper-responsiveness, we evaluated the secretion of TSLP protein from primary nasal epithelial cells (NEC) in relation to rs1837253 genotype.

Methods

Peripheral blood CD34+ cells derived from atopic and nonatopic individuals were stimulated with and/or IL-3, IL-5, or GM-CSF and assessed for Eo/B colony forming units (CFU) in both methylcellulose and nasal epithelial/CD34+ cell air-liquid interface (ALI) co-cultures, and cytokine surface receptor expression by flow cytometry. Genotyping was performed using a commercially available TaqMan® genotyping assay.

Results

TSLP preferentially enhanced IL-3/TNFα-dependent Eo/B CFU from CD34+ cells;

Eo/B CFU and TSLPR expression were significantly increased in atopic-derived CD34+ cells post TSLP- (p<0.05) and IL-3/TSLP-stimulation (p<0.001), compared to non-atopic-derived CD34+ cells;

In progenitor/NEC ALI co-cultures, secreted TSLP was biologically active, and sufficient to induce the differentiation of CD34+ cells into Eo/B CFU;

There was decreased TSLP secretion by nasal epithelial cells obtained from heterozygous (CT; 1.8-fold) and homozygous rs1837253 TSLP minor allele (TT; 2.5-fold) individuals, compared to homozygous ‘wild-type’ individuals (CC; p<0.05).

Conclusions

These studies further support the concept of in situ hemopoiesis and point out a previously unrecognized, critical role for TSLP in the development of allergic upper airway inflammation and its role in Eo/B differentiation, a link between adaptive and innate immunity. Novel functional genomics of TSLP show that the rs1837253 polymorphism may be directly involved in the regulation of TSLP secretion, providing explanations for both genetic association studies and recently reported positive clinical trials targeting TSLP in allergic asthma.

Authors’ Affiliations

(1)
Department of Medicine, McMaster University
(2)
St. Paul’s Hospital, University of British Columbia

Copyright

© Denburg et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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