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The IgE-diagnostic impact of a recombinant serine protease inhibitor from Hevea brasiliensis in latex-allergic health care workers
Clinical and Translational Allergy volume 4, Article number: P56 (2014)
Serine protease inhibitors (SPI) comprising 60-90 amino acid residues, are frequent in plants, and belong to the protein family PR-6. It has recently been shown that a SPI-variant of wheat acts as an important allergen in baker's asthma but not in persons suffering from wheat-induced food allergy. The aim of this study was to elucidate the role of a recombinant SPI-variant of Hevea brasiliensis for occupational latex allergy.
For this purpose a cDNA from Hevea brasiliensis was used to amplify the SPI-specific sequence. After identification by sequencing and transfer into the pMAL-system, the sequence was expressed as maltose-binding protein (MBP)-SPI hybrid in E.coli. The soluble purified fusion protein was biotinylated and coupled to Streptavidin-ImmunoCAP. Sera of 21 health care workers (HCW) with allergic symptoms to natural rubber latex (NRL) were tested.
Specific IgE (sIgE)-values >0.35 kUA/L were regarded as positive. Seven sera (33%) displayed SPI-sIgE (range: 0.56-13.60 kUA/L). Out of these seven sera all displayed sIgE to rHev b 6.01 (range: 1.81- 66.80 kUA/L) and with one exception also sIgE to rHev b 5 (1.86-49.6 kUA/L). Furthermore, positive sIgE to nHev b 2 (n=6/7) and rHev b 7 (n=4/7) were most frequently. No sIgE to MBP was detectable.
The results show that this SPI-variant is a further latex allergen, which is available now for extended tests, for instance on microarray platforms. The proposed name Hev b 15 is currently under review of the WHO/IUIS allergen nomenclature subcommittee. Especially in patients showing discrepancies between the sum of sIgE-values to single NRL-allergens and the IgE-value to NRL k82, Hev b 15 may be one candidate filling the gap.
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Rihs, HP., Sander, I., Heimann, H. et al. The IgE-diagnostic impact of a recombinant serine protease inhibitor from Hevea brasiliensis in latex-allergic health care workers. Clin Transl Allergy 4 (Suppl 2), P56 (2014). https://doi.org/10.1186/2045-7022-4-S2-P56
- Food Allergy
- Health Care Worker
- Serine Protease Inhibitor
- Microarray Platform