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Do hypoallergenic cats exist? -- Determination of major cat allergen Fel d 1 production in normal and hypoallergenic cat breeds

Background and aims

Due to the increasing prevalence of cat allergy the demand for so-called hypoallergenic cats and the number of respective breeders is rising. The aim of our study was to examine the hypoallergenicity of these cats, taking the major allergen Fel d 1 as a marker molecule. This molecule is an important asthma inducer and primarily produced in the sebaceous, salivary and anal glands and is distributed on the fur by licking.


We collected samples from 6 normal and 8 hypoallergenic cats by stroking with absorbent cotton over the face, chest and saliva. Allergens were extracted and Fel d 1 levels analyzed in each sample using a commercial ELISA-Kit. The allergen content of the samples was analyzed on SDS-PAGE. IgE binding activity was tested by immunoblot under reducing and non-reducing conditions using sera of cat allergic patients and monoclonal anti-Fel d 1 antibody.


Total Fel d 1 levels were reduced in sampels from the face and even more in those from the chest of hypoallergenic cats. IgE binding of human patients sera with cat samples showed that only under non-reducing conditions signals were detectable at 18 and 35kDa. Additionally, samples of normal cats showed stronger IgE binding than hypoallergenic cat samples. The monoclonal anti-Fel d 11 antibody showed stronger binding and detected two bands at 18 and 35kDa in normal cats. In contrast less intensive and only a single Fel d 11 band was detected at 18kDa in the samples from hypoallergenic cats.


Based on our data we conclude that hypoallergenic cats secrete and distribute less Fel d 1 as compared to normal cats to their fur coat. We propose that indeed, hypoallergenic cat breeds pose an attractive alternative for atopic or cat allergic patients.

Supported by the Austrian Science Fund grant F4606-B19.

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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

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Satorina, J., Szalai, K., Willensdorfer, A. et al. Do hypoallergenic cats exist? -- Determination of major cat allergen Fel d 1 production in normal and hypoallergenic cat breeds. Clin Transl Allergy 4 (Suppl 2), P11 (2014).

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