Volume 4 Supplement 2

5th International Symposium on Molecular Allergology (ISMA 2013)

Open Access

Glutathione-s-transferase is a minor allergen in birch pollen because of restricted release from hydrated pollen grains

  • Stephan Deifl1,
  • Christian Zwicker1,
  • Eva Vejvar2,
  • Claudia Kitzmüller1,
  • Gabriele Gadermaier2,
  • Birgit Nagl1,
  • Susanne Vrtala1,
  • Gerhard Zlabinger3,
  • Peter Briza2,
  • Fatima Ferreira2 and
  • Barbara Bohle1
Clinical and Translational Allergy20144(Suppl 2):O1

https://doi.org/10.1186/2045-7022-4-S2-O1

Published: 17 March 2014

Background

Recently, proteomic profiling of birch pollen detected a protein homologous to glutathione-S-transferases (GST) in prominent amounts. In mites, cockroach and fungi, GST are relevant allergens. This tempted us to investigate the allergenicity of GST from birch (bGST).

Methods

bGST was expressed in Escherichia coli, purified and characterized by mass spectrometry. BALB/c mice were immunized with bGST or Bet v 1. Antibody and T cell responses were assessed. 217 sera from birch pollen-allergic patients were tested for IgE-reactivity to bGST by ELISA. The allergenicity of bGST was evaluated with IgE-loaded rat basophil leukaemia cells (RBL) expressing the α-chain of the human receptor FcεRI. Cross-reactivity of IgE between bGST and GST from house dust mite, Der p 8, was assessed with murine and human sera in ELISA. The release kinetics of bGST and Bet v 1 from birch pollen upon hydration were studied by immunoblotting.

Results

Immunization with bGST induced specific IgE and a Th2-dominated cellular immune response comparably to immunization with Bet v 1. Only 13.4% of birch pollen-allergic patients were sensitized to bGST. In RBL assays bGST induced mediator release. GST from birch and house dust mites did not cross-react. In contrast to Bet v 1, bGST showed a limited and delayed release from hydrated birch pollen grains.

Conclusion

bGST induces specific IgE in mice but is of limited sensitizing capacity for humans. In contrast to Bet v 1, the release of bGST from hydrated pollen is restricted. Thus, the minor allergenicity of bGST may be explained by a limited exposure of patients to this protein.

Authors’ Affiliations

(1)
Department of Pathophysiology and Allergy Research, Medical University of Vienna
(2)
Department of Molecular Biology, University of Salzburg
(3)
Medical University of Vienna, Institute of Immunology

Copyright

© Deifl et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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