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  • Poster presentation
  • Open Access

Basophil response to peanut allergens in the mediterranean area

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Clinical and Translational Allergy20133 (Suppl 3) :P167

  • Published:


  • Skin Prick Test
  • Good Tolerance
  • Allergic Patient
  • Lipid Transfer Protein
  • FACSCalibur Flow Cytometer


The major peanut allergens Arah1, Arah2, and Arah3 are considered the more important sensitizers in peanut allergy, although the lipid transfer protein (LTP) Arah9 has been shown relevant in Mediterranean areas. We evaluated the basophil activation response (BAT) to different peanut allergens and the peach LTP Prup3 in patients with peanut allergy.


We included 60 subjects in different groups: Group 1, peanut and peach allergy; Group 2, peanut allergy and good tolerance to peach; Group 3, peach allergy and good tolerance to peanut; Group 4, good tolerance to peach and peanut. The diagnostic was performed by clinical history, skin prick test and/or double blind placebo controlled food challenge. BAT to Arah1, Arah2, Arah3, Arah6, rArah9 and rPrup3 was done using anti-IgE and CD63 and cells analyzed in a FACSCalibur flow cytometer.


Comparisons of BAT results between peanut allergic patients (Group 1 and 2) and controls (Group 4) showed increased basophil activation in Group 1 with Arah2 (p=0.031) and Prup3 (p=0.009) whereas Group 2 had an increase with Arah1 (p=0.016), Arah2 (p=0.001) and Arah9 (p=0.016), but not with Prup3. Comparisons with subjects allergic to peach with good tolerance to peanut (Group 3) showed a BAT increase only with Arah2 (p=0.009) in Group 2. Finally, the comparisons between groups with good tolerance to peanut (Groups 3 and 4) showed significant differences for peach allergic patients with Arah9 (p=0.004) and Prup3 (p=0.008).


The peanut allergen Arah2 is the best to discriminate peanut allergy in a Mediterranean population where LTP is highly prevalent.

Disclosure of interest

None declared.

Authors’ Affiliations

Resarch Laboratory, IMABIS Foundation-Carlos Haya Hospital/Bionand, Malaga, Spain
Allergy Service, Carlos Haya Hospital, Malaga, Spain
HAL Allergy, HAL Allergy, Leiden, the Netherlands
Centre for Plant Biotechnology and Genomics, UPM-INIA, Pozuelo de Alarcon, Spain
Division of Allergology, Paul-Ehrlich-Institut, Federal Institute for Vaccines and Biomedicines, Landen, Germany


© Mayorga et al; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.