Skip to main content
  • Poster presentation
  • Open access
  • Published:

Exercise-related anaphylaxis with food allergy to cross-reactive LTPs


In food dependent exercise-induced anaphylaxis, a food challenge followed by an exercise test, being the gold standard for diagnosis, might not be feasible under certain circumstances. Therefore, in vitro diagnostic tests can be helpful to clarify severe forms of exercise-related hypersensitivity syndromes. We report two patients with food allergy to non-specific LTPs presenting as exercise-related anaphylaxis after food intake. Our aim was to identify IgE cross-reactivities using an in-vitro depletion assay with the suspected native allergens, and an allergen microarray.


Case 1

A 25-year-old man with cholinergic urticaria and mild OAS to peach, suffered an episode of generalized urticaria and angioedema during a recreational soccer match, which was preceded by peach based soft drink ingestion.

Case 2

A 19-year-old woman with controlled asthma and allergic rhinitis to mites and grass pollens, suffered an anaphylactic shock while playing soccer. She had ingestedwalnuts 1 ½ hours before, and tomato, mango, orange and wheat bread with cheese within the previous 6 hours.

No other episodes of anaphylaxis to foods were declared in both patients.

Standard investigations included skin tests (ST) and specific IgE (sIgE). Confirmative oral challenges followed by an exercise test were precluded as case 1 suffered from cholinergic urticaria and case 2 had a life-threatening reaction.

Immunodepletion was performed with the serum of each patient using the suspected native allergen (peach and walnut, respectively) and the samples re-tested with ImmunoCAP ISAC.


In case 1, ST and ISAC confirmed IgE sensitization only to LTP components. After serum pre-incubation, there was a 100% depletion of sIgE to all cross-reactive LTPs tested in ISAC.

In case 2, ST and sIgE were positive to various fruits of Rosaceae and non Rosaceae family and nuts, including mango, orange and walnuts. ISAC detected sensitization to all LTPs tested, with species-specific sensitization to mites and grass pollens. The percentages of sIgE depletion ranged from 60% (Ara h 9) to 100% (Cor a 8).


In both patients sIgE to cross-reactivity fruit LTPs was clearly demonstrated by the in vitro methods. Exercise may be a relevant co-factor for a severe clinical presentation in LTP-related food allergy. We stress the relevance of an accurate identification of culprit allergens in life-threatening events, when re-challenge might not be feasible.

Disclosure of interest

None declared.

Author information

Authors and Affiliations


Rights and permissions

Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and permissions

About this article

Cite this article

Vieira, T., Pereira, A., Silva, D. et al. Exercise-related anaphylaxis with food allergy to cross-reactive LTPs. Clin Transl Allergy 3 (Suppl 3), P162 (2013).

Download citation

  • Published:

  • DOI: