Figure 1From: The significance of diagnosing associated clonal mast cell diseases in patients with venom-induced anaphylaxis and the role of bone marrow investigationFlow cytometric analysis of bone marrow aspirates in three cases. The mast cell frequency and phenotype as evaluated by multiparameter flow cytometry. The percentages of CD117++/CD33+ mast cells and of mast cells with aberrant phenotype CD117++/CD33+/CD25+/CD2+ in the three cases are indicated in the graph. In case 1, 0.27% of total bone marrow cells were mast cells. These could be divided, based on expression of CD25 and CD2 in two populations; normal mast cells (pink) (0.16%) and pathological mast cells with expression of CD25 (green) and CD2 (blue) (0.11%). In case 2, total mast cells were 0.09% and the majority of these were CD25+/CD2+ (0.08%). In case 3, the frequency of mast cells was below the detection limit. The antibody combination used was CD2 (FITC; DAKO) - CD25 (PE; DAKO) - CD33 (PerCP-Cy5.5; Becton-Dickinson) - CD34 (PC7; Beckman-Coulter) - CD117 (APC; Beckman-Coulter) – HLA-DR (Pacific Blue; BioLegend) – CD45 (Krome Orange; Beckman-Coulter). 500 000 cells were collected using a Canto2 flow cytometer (BD Bioscience).Back to article page