Figure 4

IgG-inhibition experiments in immunoblotting. Inhibition experiments of the IgG-binding of the rabbit anti-Sin a 2 serum (1/100000 diluted) to A) Sin a 2 and B) almond, hazelnut, pistachio and walnut protein extracts (50 μg of total protein per lane). BSA (20 μg/ml), Sin a 2 (20 μg/ml), mustard, hazelnut, pistachio or walnut protein extracts (1 mg/ml) were used as inhibitors. Molecular mass markers are displayed in the left margin.