Fig. 5
From: A novel ELISA method to determine human MrgX2 in chronic urticaria
Establishment and methodological investigation of human MrgX2-ELISA. a Double antibody sandwich screening of matched antibodies. Student’s t test (nonparametric tests) was used to determine statistical significance. b Western blot to examine the specificity of E7630 rabbit polyclonal purified antibody. c Western blot to investigate the specificity of mouse monoclonal purified antibody. d Human MrgX2-Inspection of standard curve of ELISA. e Investigation of stability of human MrgX2-ELISA. f Investigation of specificity of human MrgX2-ELISA. One-way analysis of variance (Bonferroni’s multiple comparisons test) was used to determine statistical significance. Data are expressed as mean ± SEM from at least three independent experiments. **p < .01, ***p < .001, ****p < .0001 vs. negative control