Fig. 3

Pro-inflammatory cytokines induce the expression of miR-146a in HBECs. Cultured HBECs were stimulated with shown cytokines for 48 h or left unstimulated (US). a Relative expression of miR-146a expression was measured by RT-qPCR and is shown in comparison with US cells. b Heatmap of relative mRNA expression levels of indicated genes measured by RT-qPCR. Log2 values of fold changes were mean-centered for each gene separately. c IL-8 and CXCL1 protein levels from the supernatants of cytokine stimulated HBECs measured by ELISA. a, c Data represent mean ± SEM. a–c Unpaired t-test, *P < 0.05, **P < 0.01, ***P < 0.001