Fig. 1

Western blot analysis of native sesame OAPs fraction to confirm the absence of seed storage protein traces (a) and the specific IgE (b) and IgG sensitisation pattern (c) of all included patients. a Western-Blot analysis of an intermediate purification step (before HIC) and of the final OAPs fraction (after HIC) with sera containing sIgE to sesame seed storage proteins and oleosins. Samples were separated by SDS-PAGE using a 4–12% Bis–Tris gel and blotted onto a nitrocellulose membrane. Bound IgE were detected by anti-human IgE antibodies conjugate labelled with alkaline phosphatase; PC is a oleosin-positive serum, SP is a sesame seed storage positive serum not used in this study, sera G1b_1, 4, 14, 11, 15–18, G2_12 and 10 are sera with specific IgE to sesame seed storage proteins and partly with slightly elevated sIgE levels to sesame oleosins. b IgE sensitisation pattern to sesame extract from ImmunoCAP and EUROLINE, sesame components and oleosins from sesame, walnut, soy, hazelnut and peanut expressed as EAST (Enzyme-Allergo-Sorbent Test classification)-classes and sorted by severity of symptoms to sesame, light grey: not determined. c IgG-sensitisation pattern to sesame components and oleosins from sesame, walnut, pecan and soy expressed as EUROLINE (EL)-intensities and sorted by severity of symptoms to sesame. G1a sesame allergic patients without detectable sIgE sensitisation, G1b sesame allergic patients with sIgE sensitisation, G2 sesame tolerant patients with sIgE sensitisation, PC positive control selected by sIgE to sesame oleosins, C1-3 positive controls selected by sIgE to peanut oleosins