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Table 1 Primary nasal epithelial cells of 5 non-allergic individuals were stimulated for 24 h with different TLR ligands: TLR2 (PGN: 10 μg/ml), TLR3 (Poly(I:C): 20 μg/ml), TLR4 (LPS 1 μg/ml)

From: Expression profiling and functional analysis of Toll-like receptors in primary healthy human nasal epithelial cells shows no correlation and a refractory LPS response

 

Cut off value

IMDM

Poly(I:C)

LPS

PGN

Mean

SD

Mean

SD

Mean

SD

Mean

SD

IL-1β

20

BD

 

26

28

BD

 

BD

 

IL1RA

15

198

151

713**

487

219

111

337**

179

IL-2

3

BD

 

13

15

BD

 

BD

 

IL-4

3

BD

 

13**

12

BD

 

BD

 

IL-5

2

BD

 

BD

 

BD

 

BD

 

IL-6

3

351

331

1793**

1491

264

256

548**

521

IL-7

3

24

36

132

122

34

35

45

44

IL-10

16

BD

 

BD

 

BD

 

BD

 

IL-12

8

BD

 

46

27

BD

 

BD

 

IL-13

4

BD

 

BD

 

BD

 

BD

 

IL-15

6

BD

 

28

27

BD

 

BD

 

IL-17

7

17

19

26

32

11

17

10

20

Eotaxin

6

BD

 

BD

 

BD

 

BD

 

FGF basic

15

BD

 

40**

40

BD

 

BD

 

G-CSF

2

72

95

655**

700

BD

 

132

179

GMCSF

4

36

74

58

148

18

47

20

48

INF γ

80

BD

 

BD

16

BD

 

BD

 

IP-10

24

BD

 

1982**

1666

BD

 

29

27

MCP 1

6

219

111

414

456

204

107

278

216

MIP1α

2

8

16

1122

2376

4

10

4

11

MIP1β

38

BD

 

930**

1321

BD

 

BD

 

MIG

4

5

11

23

22

BD

 

BD

 

RANTES

5

19

23

2120**

1516

18

21

25

25

TNFα

10

BD

 

43

68

BD

 

BD

 

VEGF

9

191

163

482**

508

161

142

262**

189

EGF

1

BD

 

5

10

BD

 

1

4

HGF

66

BD

 

31

28

BD

 

BD

 

IL-2R

7

70

52

632**

491

65

36

99

63

IFNα

2

14

21

39

39

10

18

22

20

  1. Cell free supernatants were analysed using a Luminex array. Concentrations are presented as average of triplicates of 5 different patients in pg/ml. The detection limits are shown as cut off value
  2. SD Standard deviation, BD below detection level
  3. ** P < 0.05