Volume 4 Supplement 2

5th International Symposium on Molecular Allergology (ISMA 2013)

Open Access

Peanut panallergen Ara h 8 IgE and IgG4 epitopes

  • Barry Hurlburt1,
  • Hsiaopo Cheng2,
  • Lesa Offermann3,
  • Maksymilian Chruszcz3,
  • Alexandra Santos4,
  • Gideon Lack4 and
  • Soheila Maleki2
Clinical and Translational Allergy20144(Suppl 2):P30

https://doi.org/10.1186/2045-7022-4-S2-P30

Published: 17 March 2014

Background

Ara h 8 is hypothesized to be the panallergen responsible for oral allergy syndrome between birch pollen (Bet v 1) and peanut. We recently determined the crystal structure of Ara h 8. In this work, we probed microarrays of peptides with peanut allergic and peanut sensitized patient sera for IgE and IgG4 reactivity.

Methods

15-mer peptides that were offset by 5 amino acids were printed to glass. Patient sera was incubated with the slides. IgE and IgG4 binding was detected with combinations of secondary and fluorescently-labeled tertiary antibodies. The linear epitopes identified were mapped on the 3-D structure and compared with those of birch pollen protein Bet v 1.

Results

The majority of the Ara h 8 IgE epitopes mapped in this work align with those identified with Bet v 1. Considerably more IgG4 epitopes than IgE epitopes were found. Peanut allergic sera were more reactive with regard to IgE and IgG4 than peanut sensitized sera.

Conclusion

Our results support both the hypothesis that Ara h 8 could be contributing to oral allergy syndrome between birch pollen and peanut.

Authors’ Affiliations

(1)
U.S. Department of Agriculture, Southern Regional Research Center
(2)
U.S. Department of Agriculture, Food Allergy Research Unit
(3)
University of South Carolina, Chemistry and Biochemistry
(4)
King's College, Paediatric Allergy

Copyright

© Hurlburt et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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