Volume 1 Supplement 1

Food Allergy and Anaphylaxis Meeting 2011

Open Access

Identification of marker allergens in Brazilian soy allergic patients

  • Renata Cocco1,
  • Dirceu Solé1,
  • Marcia Mallozi1,
  • Charles Naspitz1,
  • Fredrik Bernhardsson2,
  • Sigrid Sjolander2,
  • Maryam Poorafshar2 and
  • Anita Kober2
Clinical and Translational Allergy20111(Suppl 1):P72

DOI: 10.1186/2045-7022-1-S1-P72

Published: 12 August 2011

Background

Soy allergy is established as one of the most common food allergies. Therefore, we decided to analyze whether there are specific protein markers among Brazilian soy allergic patients, using microarray techniques.

Method

Thirteen patients presenting with immediate symptoms after ingestion of soy formula and IgE reactivity to soy extract were selected after failing oral food challenges. Sixteen patients with no symptoms (negative soy challenge), but presenting with positive specific IgE to soy (ImmunoCAP > 0,35kU/L) were used as controls. In order to allow for simultaneous measurement of IgE responses, a number of purified proteins and protein mixes were used for analysis in a multiplexed capillary-flow based microarray assay.

Results

Significant differences between children with symptoms and without symptoms were found for βconglycinin (Gly m 5) and glycinin (Gly m 6), the storage proteins of soybean. β-conglycinin showed higher response and glycinin, lower response by the tested patients.

Conclusion

The highest and most prevalent IgE reacitivity in the group of children with soy allergy was directed to the two storage proteins of soy, β-conglycinin and glycinin. Both proteins appear to be good marker allergens for soy allergy in Brazilian children.

Authors’ Affiliations

(1)
Federal University of São Paulo, Pediatrics
(2)
Phadia AB

Copyright

© Cocco et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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