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Table 2 Laboratory data of anaphylactic patients and healthy subjects

From: Chemokines during anaphylaxis: the importance of CCL2 and CCL2-dependent chemotactic activity for basophils

Variables median (IQR) Patients with an acute episode of anaphylaxis (n = 49)a Healthy subjects (n = 28)b P-value
Acute sampling Convalescent sampling Acute vs. convalescent Acute vs. healthy subjects Convalescent vs. healthy subjects
Serum chemokines (pg/ml)
 CCL2/MCP-1 514 (561) 284 (128) 279 (147) < 0.0001 < 0.0001 0.9726
 CCL7/MCP-3 8.9 (2.9) 8.5 (2.1) 8.1 (1.4) 0.7549 0.1636 0.1038
 CCL8/MCP-2 73 (56) 69 (35) 70 (19) 0.1579 0.1425 0.4226
 CCL13/MCP-4 113 (81) 104 (88) 123 (103) 0.9032 0.3367 0.7648
 CCL11/Eotaxin 200 (174) 211 (174) 160 (60) 0.2710 0.1641 0.0555
 CCL24/Eotaxin-2 1124 (1377) 946 (1036) 1159 (1561) 0.0940 0.8665 0.5919
 CCL26/Eotaxin-3 1903 (88) 1898 (181) 1877 (155) 0.0412 0.1746 0.4640
 CCL5/Rantes 40,907 (34,588) 41,795 (27,011) 46,993 (33,574) 0.6669 0.7485 0.9659
 CCL17/TARC 342 (194) 303 (203) 344 (180) 0.0148 0.7093 0.1931
 CCL21/SLC 1179 (1193) 1099 (645) 938 (417) 0.2206 0.3376 0.4175
 CCL22/MDC 666 (321) 717 (251) 766 (260) 0.7045 0.8014 0.5408
Serum tryptase
 µg/l 12.9 (10.6) 5.3 (2.0) 3.3 (2.0) < 0.0001 < 0.0001 0.0005
 > 11.4 µg/l, n (%) 18 (60) 1 (3.3) 0 (0) < 0.0001 < 0.0001 1
Absolute cell count (cells/µl)
 Basophils 5.0 (7.3) 19.1 (10.9) < 0.0001
 Monocytes 396 (163) 441 (108) 0.8417
 Lymphocytes 1378 (1219) 1461 (470) 0.3904
 PMNs 3881 (2873) 2875 (1144) 0.0388
Gene expression in whole blood (fold change)
 FCER1A 0.11 (0.24) 0.40 (0.64) 0.0002
 CPA3 0.22 (0.51) 0.72 (1.16) 0.0002
 HDC 0.11 (0.30) 0.38 (0.57) 0.0018
Serum of patients used for in vitro migration assays
 Tryptase (µg/l) 22.6 (16.5) 6.4 (6.2) 4.3 (5.8) 0.0002 0.0003 0.5358
 > 11.4 µg/l, n (%) 8 (100) 0 (0) 0 (0) 0.0002 0.0002 1
 CCL2 (pg/ml) 1964 (1114) 278 (175) 302 (76) 0.0002 0.0003 0.3969
Basophils of healthy donors used for the in vitro migration assays
 Absolute count (cells/µl) 18.3 (6.3)
 CCR2 expression MFI 353 (422)
 % CCR2 + basophils 94 (27)
  1. PMNs polymorphonuclear leukocytes, FCER1A α subunit of the high-affinity IgE receptor, CPA3 carboxypeptidase A3, HDC histidine decarboxylase, CCR2 C-C motif chemokine receptor 2, MFI mean fluorescence intensity
  2. P values were Bonferroni-corrected, and P-value < 0.0026 was accepted as significant. Statistically significant P values are presented in boldface
  3. aIn 30 patients, we measured serum chemokines and tryptase, and in 26 patients, we measured blood absolute cell counts and gene expression (of those 41 patients, 15 were in both subgroups); serum samples from 8 patients were used for in vitro migration experiments
  4. bIn 20 healthy subjects, we measured serum chemokines and tryptase; basophils from 8 healthy subjects were used for in vitro migration experiments