|
Variables median (IQR)
|
Patients with an acute episode of anaphylaxis (n = 49)a
|
Healthy subjects (n = 28)b
|
P-value
|
|---|
|
Acute sampling
|
Convalescent sampling
|
Acute vs. convalescent
|
Acute vs. healthy subjects
|
Convalescent vs. healthy subjects
|
|---|
|
Serum chemokines (pg/ml)
|
|
CCL2/MCP-1
|
514 (561)
|
284 (128)
|
279 (147)
|
< 0.0001
|
< 0.0001
|
0.9726
|
|
CCL7/MCP-3
|
8.9 (2.9)
|
8.5 (2.1)
|
8.1 (1.4)
|
0.7549
|
0.1636
|
0.1038
|
|
CCL8/MCP-2
|
73 (56)
|
69 (35)
|
70 (19)
|
0.1579
|
0.1425
|
0.4226
|
|
CCL13/MCP-4
|
113 (81)
|
104 (88)
|
123 (103)
|
0.9032
|
0.3367
|
0.7648
|
|
CCL11/Eotaxin
|
200 (174)
|
211 (174)
|
160 (60)
|
0.2710
|
0.1641
|
0.0555
|
|
CCL24/Eotaxin-2
|
1124 (1377)
|
946 (1036)
|
1159 (1561)
|
0.0940
|
0.8665
|
0.5919
|
|
CCL26/Eotaxin-3
|
1903 (88)
|
1898 (181)
|
1877 (155)
|
0.0412
|
0.1746
|
0.4640
|
|
CCL5/Rantes
|
40,907 (34,588)
|
41,795 (27,011)
|
46,993 (33,574)
|
0.6669
|
0.7485
|
0.9659
|
|
CCL17/TARC
|
342 (194)
|
303 (203)
|
344 (180)
|
0.0148
|
0.7093
|
0.1931
|
|
CCL21/SLC
|
1179 (1193)
|
1099 (645)
|
938 (417)
|
0.2206
|
0.3376
|
0.4175
|
|
CCL22/MDC
|
666 (321)
|
717 (251)
|
766 (260)
|
0.7045
|
0.8014
|
0.5408
|
|
Serum tryptase
|
|
µg/l
|
12.9 (10.6)
|
5.3 (2.0)
|
3.3 (2.0)
|
< 0.0001
|
< 0.0001
|
0.0005
|
|
> 11.4 µg/l, n (%)
|
18 (60)
|
1 (3.3)
|
0 (0)
|
< 0.0001
|
< 0.0001
|
1
|
|
Absolute cell count (cells/µl)
|
|
Basophils
|
5.0 (7.3)
|
19.1 (10.9)
|
–
|
< 0.0001
|
–
|
–
|
|
Monocytes
|
396 (163)
|
441 (108)
|
–
|
0.8417
|
–
|
–
|
|
Lymphocytes
|
1378 (1219)
|
1461 (470)
|
–
|
0.3904
|
–
|
–
|
|
PMNs
|
3881 (2873)
|
2875 (1144)
|
–
|
0.0388
|
–
|
–
|
|
Gene expression in whole blood (fold change)
|
|
FCER1A
|
0.11 (0.24)
|
0.40 (0.64)
|
–
|
0.0002
|
–
|
–
|
|
CPA3
|
0.22 (0.51)
|
0.72 (1.16)
|
–
|
0.0002
|
–
|
–
|
|
HDC
|
0.11 (0.30)
|
0.38 (0.57)
|
–
|
0.0018
|
–
|
–
|
|
Serum of patients used for in vitro migration assays
|
|
Tryptase (µg/l)
|
22.6 (16.5)
|
6.4 (6.2)
|
4.3 (5.8)
|
0.0002
|
0.0003
|
0.5358
|
|
> 11.4 µg/l, n (%)
|
8 (100)
|
0 (0)
|
0 (0)
|
0.0002
|
0.0002
|
1
|
|
CCL2 (pg/ml)
|
1964 (1114)
|
278 (175)
|
302 (76)
|
0.0002
|
0.0003
|
0.3969
|
|
Basophils of healthy donors used for the in vitro migration assays
|
|
Absolute count (cells/µl)
|
–
|
–
|
18.3 (6.3)
|
–
|
–
|
–
|
|
CCR2 expression MFI
|
–
|
–
|
353 (422)
|
–
|
–
|
–
|
|
% CCR2 + basophils
|
–
|
–
|
94 (27)
|
–
|
–
|
–
|
- PMNs polymorphonuclear leukocytes, FCER1A α subunit of the high-affinity IgE receptor, CPA3 carboxypeptidase A3, HDC histidine decarboxylase, CCR2 C-C motif chemokine receptor 2, MFI mean fluorescence intensity
- P values were Bonferroni-corrected, and P-value < 0.0026 was accepted as significant. Statistically significant P values are presented in boldface
- aIn 30 patients, we measured serum chemokines and tryptase, and in 26 patients, we measured blood absolute cell counts and gene expression (of those 41 patients, 15 were in both subgroups); serum samples from 8 patients were used for in vitro migration experiments
- bIn 20 healthy subjects, we measured serum chemokines and tryptase; basophils from 8 healthy subjects were used for in vitro migration experiments
