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Table 2 Laboratory data of anaphylactic patients and healthy subjects

From: Chemokines during anaphylaxis: the importance of CCL2 and CCL2-dependent chemotactic activity for basophils

Variables median (IQR)

Patients with an acute episode of anaphylaxis (n = 49)a

Healthy subjects (n = 28)b

P-value

Acute sampling

Convalescent sampling

Acute vs. convalescent

Acute vs. healthy subjects

Convalescent vs. healthy subjects

Serum chemokines (pg/ml)

 CCL2/MCP-1

514 (561)

284 (128)

279 (147)

< 0.0001

< 0.0001

0.9726

 CCL7/MCP-3

8.9 (2.9)

8.5 (2.1)

8.1 (1.4)

0.7549

0.1636

0.1038

 CCL8/MCP-2

73 (56)

69 (35)

70 (19)

0.1579

0.1425

0.4226

 CCL13/MCP-4

113 (81)

104 (88)

123 (103)

0.9032

0.3367

0.7648

 CCL11/Eotaxin

200 (174)

211 (174)

160 (60)

0.2710

0.1641

0.0555

 CCL24/Eotaxin-2

1124 (1377)

946 (1036)

1159 (1561)

0.0940

0.8665

0.5919

 CCL26/Eotaxin-3

1903 (88)

1898 (181)

1877 (155)

0.0412

0.1746

0.4640

 CCL5/Rantes

40,907 (34,588)

41,795 (27,011)

46,993 (33,574)

0.6669

0.7485

0.9659

 CCL17/TARC

342 (194)

303 (203)

344 (180)

0.0148

0.7093

0.1931

 CCL21/SLC

1179 (1193)

1099 (645)

938 (417)

0.2206

0.3376

0.4175

 CCL22/MDC

666 (321)

717 (251)

766 (260)

0.7045

0.8014

0.5408

Serum tryptase

 µg/l

12.9 (10.6)

5.3 (2.0)

3.3 (2.0)

< 0.0001

< 0.0001

0.0005

 > 11.4 µg/l, n (%)

18 (60)

1 (3.3)

0 (0)

< 0.0001

< 0.0001

1

Absolute cell count (cells/µl)

 Basophils

5.0 (7.3)

19.1 (10.9)

–

< 0.0001

–

–

 Monocytes

396 (163)

441 (108)

–

0.8417

–

–

 Lymphocytes

1378 (1219)

1461 (470)

–

0.3904

–

–

 PMNs

3881 (2873)

2875 (1144)

–

0.0388

–

–

Gene expression in whole blood (fold change)

 FCER1A

0.11 (0.24)

0.40 (0.64)

–

0.0002

–

–

 CPA3

0.22 (0.51)

0.72 (1.16)

–

0.0002

–

–

 HDC

0.11 (0.30)

0.38 (0.57)

–

0.0018

–

–

Serum of patients used for in vitro migration assays

 Tryptase (µg/l)

22.6 (16.5)

6.4 (6.2)

4.3 (5.8)

0.0002

0.0003

0.5358

 > 11.4 µg/l, n (%)

8 (100)

0 (0)

0 (0)

0.0002

0.0002

1

 CCL2 (pg/ml)

1964 (1114)

278 (175)

302 (76)

0.0002

0.0003

0.3969

Basophils of healthy donors used for the in vitro migration assays

 Absolute count (cells/µl)

–

–

18.3 (6.3)

–

–

–

 CCR2 expression MFI

–

–

353 (422)

–

–

–

 % CCR2 + basophils

–

–

94 (27)

–

–

–

  1. PMNs polymorphonuclear leukocytes, FCER1A α subunit of the high-affinity IgE receptor, CPA3 carboxypeptidase A3, HDC histidine decarboxylase, CCR2 C-C motif chemokine receptor 2, MFI mean fluorescence intensity
  2. P values were Bonferroni-corrected, and P-value < 0.0026 was accepted as significant. Statistically significant P values are presented in boldface
  3. aIn 30 patients, we measured serum chemokines and tryptase, and in 26 patients, we measured blood absolute cell counts and gene expression (of those 41 patients, 15 were in both subgroups); serum samples from 8 patients were used for in vitro migration experiments
  4. bIn 20 healthy subjects, we measured serum chemokines and tryptase; basophils from 8 healthy subjects were used for in vitro migration experiments
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Clinical and Translational Allergy

ISSN: 2045-7022

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