Fig. 4

a–c Effects of CCL2 and acute and convalescent serum from patients with an anaphylactic episode on basophil in vitro migration. Basophil migration after 150 min of incubation: a in the presence of rCCL2 in the lower wells and after CCL2 blocking and b in the presence of acute or convalescent serum in the lower wells and after CCL2 blocking. c Time course of basophil migration (after 30, 60, 90, 120, and 150 min of incubation) in the presence of acute and convalescent serum in the lower wells. All experiments were independently performed in triplicate, with a basophils from 5 healthy donors; b basophils from the same 5 healthy donors and serum samples from 5 patients and c basophils from 3 healthy donors and serum samples from 3 patients (details in Additional file 1: Table S1). HBSS Hank’s balanced salt solution