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Fig. 2 | Clinical and Translational Allergy

Fig. 2

From: Defining lipids and T cell receptors involved in the intrinsic allergenicity of nut proteins

Fig. 2

Fractionation of Brazil nut polar lipid (PL) by TLC (a) and in vitro TCR specific activation (b). IL-2 release of co-culture containing MUTZ3 and pMJA219 (TRAV10/TRBV25), pMJA290, 295 and 297 Jurkat 76 (J76) transient sequences experiments using nut lipid fractionated polar lipids (PL). Molecular standards on TLC: PC: Phosphatidylcholine; PE: Phosphatidylethanolamine; PG: Phosphatidylglycerol; PA: Phosphatidic acid; PS: Phosphatidylserine; DGDG: Digalactosyl Diacylglycerol. The identity of PL1, PL2 and PL3 as PC, PI and PE respectively was confirmed by ESI-MS/MS. All experiments were set up in triplicate wells and repeated three times. Error bars indicate standard deviation. P values indicate significance level as assessed by T test (*p < 0.05)

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