Fig. 4From: Reduced expression of miR-146a in human bronchial epithelial cells alters neutrophil migrationmiR-146a inhibits the expression of pro-inflammatory chemokines IL-8 and CXCL1 and moderately upregulates interferon response genes. HBECs were transfected with miR-146a or control mimic and 24 h later stimulated with indicated cytokines or left unstimulated (US) for 48 h until harvesting. Relative miR-146a expression (a) and mRNA (b–e) expression was measured by RT-qPCR. (f) Protein levels of IL-8 and CXCL1 from the supernatants of transfected and stimulated HBECs were measured by ELISA. Data represent mean ± SEM. Unpaired t-test, *P < 0.05, **P < 0.01, ***P < 0.001Back to article page