Fig. 4

Induction of IgE production by N-terminal 32-residue region of Der p 24 in Balb/c mice. a Experimental design. Postmmunization indirect ELISA detection of specific IgG (b) and IgE (c) antibodies against the conjugate. d IgE-western blot of rDer p 24 with sera from the OVA-Der p 24(1–32aa) (m1–m3 represent three different individuals) and OVA (c1 and c2 represent two different individuals) groups. e Significantly greater RBL-2H3 cell ß-hexosaminidase release was induced by sera from the OVA-Der p24(1–32aa) group than that induced by sera from the OVA and PBS groups. f Light photomicrographs of H&E-stained sections of lung tissues in mice challenged with OVA-Der p24(1–32aa), OVA, or PBS (× 100 magnification). P values < 0.05 were considered significant