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Fig. 2 | Clinical and Translational Allergy

Fig. 2

From: Identification of immunodominant IgE binding epitopes of Der p 24, a major allergen of Dermatophagoides pteronyssinus

Fig. 2

Localization of an IgE epitope of Der p 24 to the N-terminus. a Schematic representation of truncated Der p 24 fragments used for IgE epitope mapping. b SDS-PAGE analysis of rDer p 24 and five truncated forms of rDer p 24 after Ni-NTA resin purification. The gel was stained with Coomassie Brilliant Blue. c IgE binding activity of truncated Der p 24 fragments determined by IgE-western blots with pooled sera from 8 HDM-allergic patients

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