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Fig. 1 | Clinical and Translational Allergy

Fig. 1

From: Identification of immunodominant IgE binding epitopes of Der p 24, a major allergen of Dermatophagoides pteronyssinus

Fig. 1

Cloning, recombinant expression, and allergenic identification of Der p 24. A Sequence analysis of Der p 24 protein. BLAST homology comparison of Der p 24 and Der f 24 (GenBank No. AJK91617.1). After codon optimization, synthesized Der p 24 cDNA was subcloned into pET expression system to generate Der p 24 (theoretical molecular mass = 14 kDa; GenBank accession no. KP893174.1). B SDS-PAGE analysis of purified rDer p 24 stained with Coomassie brilliant blue. C Peptide coverage of rDer p 24 based on MS analysis: 10–28aa peptide data shown in a and 79–90aa peptide data shown in b. D IgE binding activity determined by IgE-western blots of rDer p 24 with individual sera from 8 HDM-allergic patients (a) and 8 healthy non-allergic individuals (b)

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