Fig. 2
From: Skin microdialysis: methods, applications and future opportunities—an EAACI position paper
An example of the SMD procedure. a Priming of microdialysis probes prior to insertion, b topical application of local anesthesia, c intradermal insertion of guide cannulas, d introduction of probes through the guide cannulas, e SMD setting and basal sampling, f skin challenge, g skin site before challenge, h wheal and flare reaction in response to intradermal challenge, i collection of dialysates—here in microtubes, j alternative collection of dialysates—here directly into sampling wells. Please refer to the SOPs (in vivo SMD SOP and ex vivo SMD SOP, provided as Additional files 1 and 2, respectively) for a detailed description of the SMD procedure. Pictures are reproduced with kind permission from Line Kring Tannert and Marcus Maurer