Fig. 3From: Evaluation of basophil activation caused by transgenic rice seeds expressing whole T cell epitopes of the major Japanese cedar pollen allergensMeasurement of the percentage of CD203c-positive activated basophils. One example of the basophil activation test in this study. Allergens examined in the basophil activation test were a PBS (negative control), b anti-IgE antibody (positive control), c Cry j 1 and Cry j 2 (allergen components of the conventional immunotherapy), d WT-rice seed extract, and e Tg-rice seed extract. The threshold for CD203c-positivity was set at less than 5% of activated cells in the negative control. The upper region of the threshold defines CD203c-positive activated basophils and the percentage of those cells was measured by the flow cytometerBack to article page