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Fig. 1 | Clinical and Translational Allergy

Fig. 1

From: Evaluation of basophil activation caused by transgenic rice seeds expressing whole T cell epitopes of the major Japanese cedar pollen allergens

Fig. 1

Allergen-specific lymphocyte stimulation test to determine the allergen concentration in the basophil activation test. Several dilutions of WT- or Tg-rice seed extract, and Cry j 1 and Cry j 2 were added to PBMCs separated from blood samples from the first three patients with JC pollinosis. Allergen-specific lymphocyte proliferative responses to those allergens were determined using an in vitro 3H-thymidine incorporation assay. The proliferation of allergen-specific lymphocytes following stimulation with 1:40 dilution of Tg-rice seed extract was almost equivalent to the proliferation following stimulation with the mixture consisting of Cry j 1 and Cry j 2 (both at 1.25 µg/mL)

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Clinical and Translational Allergy

ISSN: 2045-7022

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