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Fig. 1 | Clinical and Translational Allergy

Fig. 1

From: The antimicrobial protein S100A12 identified as a potential autoantigen in a subgroup of atopic dermatitis patients

Fig. 1

Study design and method overview. a In the initial untargeted screening, a subset of 80 subjects was analyzed for IgG-reactivity towards 1152 protein fragments using planar antigen arrays. b In the targeted analysis phase, the complete cohort of 257 subjects was profiled on antigen suspension bead arrays including 148 protein fragments selected from the screening as well as from literature (see Additional file 1: Table S1). Planar and bead-based arrays included protein fragments that were either spotted on the array glass slides (a) or coupled to the beads (b). Antigens were human protein fragments expressed with an N-terminal hexa-histidine albumin binding protein (His6-ABP) tag. Arrays were incubated with plasma and autoantibodies detected using labeled anti-human IgG. HC healthy controls, AD atopic dermatitis, mAD moderate AD, sAD severe AD

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