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Fig. 2 | Clinical and Translational Allergy

Fig. 2

From: Calu-3 epithelial cells exhibit different immune and epithelial barrier responses from freshly isolated primary nasal epithelial cells in vitro

Fig. 2

Effect of House dust mite extract on epithelial cells in vitro. Primary nasal epithelial cells (n = 5) and Calu-3 epithelial cells (n = 3) at air–liquid interface were stimulated for 4 h with different concentrations of house dust mite (HDM) extract. a Evaluation of trans-epithelial electrical resistance (TER) for 4 h of Calu-3 epithelial cell cultures. FD4 permeability of Calu-3 epithelial cells at time point 4 h. b Evaluation of TER for 4 h of primary nasal epithelial cells from heathy controls. FD4 permeability of primary nasal epithelial cells at time point 4 h. c Concentration of IL-8 in the supernatant of HDM stimulated Calu-3 or primary nasal epithelial cell cultures. Data presented as mean ± SEM. One-way ANOVA with post hoc test. *p < 0.05; **p < 0.01; ***p < 0.001; ns not significant

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