Fig. 2From: Calu-3 epithelial cells exhibit different immune and epithelial barrier responses from freshly isolated primary nasal epithelial cells in vitroEffect of House dust mite extract on epithelial cells in vitro. Primary nasal epithelial cells (n = 5) and Calu-3 epithelial cells (n = 3) at air–liquid interface were stimulated for 4 h with different concentrations of house dust mite (HDM) extract. a Evaluation of trans-epithelial electrical resistance (TER) for 4 h of Calu-3 epithelial cell cultures. FD4 permeability of Calu-3 epithelial cells at time point 4 h. b Evaluation of TER for 4 h of primary nasal epithelial cells from heathy controls. FD4 permeability of primary nasal epithelial cells at time point 4 h. c Concentration of IL-8 in the supernatant of HDM stimulated Calu-3 or primary nasal epithelial cell cultures. Data presented as mean ± SEM. One-way ANOVA with post hoc test. *p < 0.05; **p < 0.01; ***p < 0.001; ns not significantBack to article page