Fig. 5From: Maternal exposure to farming environment protects offspring against allergic diseases by modulating the neonatal TLR-Tregs-Th axisThe percentage of division of CD4 + CD25- T effector cells with or without CD4 + CD25 + Tcells. CD4 + CD25- T cells were labeled using Cell Trace™ CFSE Cell Proliferation Kit before culture and cell division percentage was measured by flow cytometry after culture. a CBMC gate; b CD4 + CFSE + gate; c the percentage of newly divided CD4 + CD25-T cells occupied 81.1% of the total effector cells after stimulated with PHA without CD4 + CD25 + T reg cells; d the percentage of newly divided CD4 + CD25- T cells occupied 50.1% of the total effector cells after stimulated with PHA when cocultured with CD4 + CD25 + T reg cells. n = 8. The gating strategy used is illustrated in Additional file 1: S3Back to article page