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Table 1 Methods for identifying mite allergens in the discovery phase

From: Consideration of methods for identifying mite allergens

Authors Methods PubMed ID Allergena Year
Aki et al. [28] cDNA library screen 7622766 Der f 10 1995
Ferrandiz et al. [18] 1D SDS-PAGE/western blotting 8556562 Der s 1 and 2 1995
Fujikawa et al. [25] cDNA library screen 8649452 Der f 14 1996
King et al. [6] Chromatography (protease activity) 8876548 Der p 9 1996
Morgan et al. [16] IEF, 1D SDS-PAGE/western blotting 9275144 Eur m 2 1997
Wey et al. [53] Chromatography (mAB) 10592827 94kd IgE binding protein 1997
Le Mao et al. [24] 2D SDS-PAGE/western blotting 9802372 Der f 14, Der f 15 1998
Tsai et al. [61] Chromatography (mAB) 9723675 Der f 11 1998
Yi et al. [15] 1D SDS-PAGE/western blotting 10697258 Blo t IgE binding proteins 1999
Binder et al. [44] cDNA library screen 11673567 Plo i 1 2001
McCall et al. [62] 1D SDS-PAGE/western blotting 11292526 Der f 15 2001
Weber et al. [37] 1D SDS-PAGE/western blotting 12847483 Der f 18 2003
Huntley et al. [45] 2D SDS-PAGE/western blotting 15679630 Pso o 10, 11 and 14 2004
Angus et al. [31] and Gao et al. [63] EST screen 15651897 Blo t 21 2004
Harris et al. [21] Protease activity screen 15489163 Der p 1 2004
Weghofer et al. [29] cDNA library screen 18445190 Der p 21 2008
Weghofer et al. [30] cDNA library screen 23460742 Der p 23 2013
An et al. [27] Chromatography, 1 and 2D SDS-PAGE/western blotting 23481662 Der f 25, 28, 29, 30 2013
Chan et al. [32] Sequence mining, 2D SDS-PAGE/western blotting 25445830 Der f 24 2015
Martins et al. [26] IEF, 1 and 2D SDS-PAGE/western blotting 26015775 Der p IgE binding proteins 2015
Choopong et al. [43] 1 and 2D SDS-PAGE/western blotting 26754146 Aconitate hydrase 2016
  1. aIf a known allergen was identified in an unbiased screen, this was still considered a discovery attempt