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Table 1 Methods for identifying mite allergens in the discovery phase

From: Consideration of methods for identifying mite allergens

Authors

Methods

PubMed ID

Allergena

Year

Aki et al. [28]

cDNA library screen

7622766

Der f 10

1995

Ferrandiz et al. [18]

1D SDS-PAGE/western blotting

8556562

Der s 1 and 2

1995

Fujikawa et al. [25]

cDNA library screen

8649452

Der f 14

1996

King et al. [6]

Chromatography (protease activity)

8876548

Der p 9

1996

Morgan et al. [16]

IEF, 1D SDS-PAGE/western blotting

9275144

Eur m 2

1997

Wey et al. [53]

Chromatography (mAB)

10592827

94kd IgE binding protein

1997

Le Mao et al. [24]

2D SDS-PAGE/western blotting

9802372

Der f 14, Der f 15

1998

Tsai et al. [61]

Chromatography (mAB)

9723675

Der f 11

1998

Yi et al. [15]

1D SDS-PAGE/western blotting

10697258

Blo t IgE binding proteins

1999

Binder et al. [44]

cDNA library screen

11673567

Plo i 1

2001

McCall et al. [62]

1D SDS-PAGE/western blotting

11292526

Der f 15

2001

Weber et al. [37]

1D SDS-PAGE/western blotting

12847483

Der f 18

2003

Huntley et al. [45]

2D SDS-PAGE/western blotting

15679630

Pso o 10, 11 and 14

2004

Angus et al. [31] and Gao et al. [63]

EST screen

15651897

Blo t 21

2004

Harris et al. [21]

Protease activity screen

15489163

Der p 1

2004

Weghofer et al. [29]

cDNA library screen

18445190

Der p 21

2008

Weghofer et al. [30]

cDNA library screen

23460742

Der p 23

2013

An et al. [27]

Chromatography, 1 and 2D SDS-PAGE/western blotting

23481662

Der f 25, 28, 29, 30

2013

Chan et al. [32]

Sequence mining, 2D SDS-PAGE/western blotting

25445830

Der f 24

2015

Martins et al. [26]

IEF, 1 and 2D SDS-PAGE/western blotting

26015775

Der p IgE binding proteins

2015

Choopong et al. [43]

1 and 2D SDS-PAGE/western blotting

26754146

Aconitate hydrase

2016

  1. aIf a known allergen was identified in an unbiased screen, this was still considered a discovery attempt