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Table 4 Comparison of peanut allergen sIgE measured with three methods: ALFA, ImmunoCAP®, ISAC®

From: 6th International Symposium on Molecular Allergology (ISMA)

Methods

Allergens

Concordance (%)

Positive/negative discordance

Ideal

Good

Poor

None

ALFA® and singleplex ImmunoCAP®

Ara h 2

26

63

11

0

0

 

Ara h 1

41

24

12

24

9, with 4 ALFA positive, ImmunoCAP negative, and 1 ALFA negative, ImmunoCAP positive

 

Ara h 9

22

44

28

6

7, with 5 ALFA positive, ImmunoCAP negative, and 2 ALFA negative, ImmunoCAP positive

 

CCD

53

33

13

0

4, all ALFA positive, ImmunoCAP negative

 

Peanut extract

17

41

41

6

0

ALFA® and ISAC® microarray

Ara h 2

42

32

16

11

0

 

Ara h 6

16

58

11

16

0

 

Ara h 1

17

33

28

22

9, all ALFA positive, ISAC negative (4 ImmunoCAP positive, 4 ImmunoCAP negative, 1 not assayed with ImmunoCAP)

 

Ara h 9

44

17

28

11

9, all ALFA positive, ISAC negative (4 ImmunoCAP positive, 5 ImmunoCAP negative)

 

CCD

33

39

22

6

10, all ALFA positive, ISAC negative (4 ImmunoCAP positive, 4 ImmunoCAP negative, 2 not assayed with ImmunoCAP)

  1. For each method, sIgE results were converted in 7 classes (0 to 6) based on the intensity of the measured signal. Ideal concordance was defined as two same-class results, good concordance as adjacent class results, poor concordance as a difference of 2 classes, and no concordance when results differed by more than 2 classes