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Table 4 Comparison of peanut allergen sIgE measured with three methods: ALFA, ImmunoCAP®, ISAC®

From: 6th International Symposium on Molecular Allergology (ISMA)

Methods Allergens Concordance (%) Positive/negative discordance
Ideal Good Poor None
ALFA® and singleplex ImmunoCAP® Ara h 2 26 63 11 0 0
  Ara h 1 41 24 12 24 9, with 4 ALFA positive, ImmunoCAP negative, and 1 ALFA negative, ImmunoCAP positive
  Ara h 9 22 44 28 6 7, with 5 ALFA positive, ImmunoCAP negative, and 2 ALFA negative, ImmunoCAP positive
  CCD 53 33 13 0 4, all ALFA positive, ImmunoCAP negative
  Peanut extract 17 41 41 6 0
ALFA® and ISAC® microarray Ara h 2 42 32 16 11 0
  Ara h 6 16 58 11 16 0
  Ara h 1 17 33 28 22 9, all ALFA positive, ISAC negative (4 ImmunoCAP positive, 4 ImmunoCAP negative, 1 not assayed with ImmunoCAP)
  Ara h 9 44 17 28 11 9, all ALFA positive, ISAC negative (4 ImmunoCAP positive, 5 ImmunoCAP negative)
  CCD 33 39 22 6 10, all ALFA positive, ISAC negative (4 ImmunoCAP positive, 4 ImmunoCAP negative, 2 not assayed with ImmunoCAP)
  1. For each method, sIgE results were converted in 7 classes (0 to 6) based on the intensity of the measured signal. Ideal concordance was defined as two same-class results, good concordance as adjacent class results, poor concordance as a difference of 2 classes, and no concordance when results differed by more than 2 classes